Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1986-02-07
1989-08-08
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 26, 536 27, 536 28, 935 88, 935 77, C12Q 168, G01N 3350, C07H 2100
Patent
active
048552259
ABSTRACT:
A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.
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Connell Charles R.
Fung Steven
Haugland Richard P.
Menchen Steven M.
Woo Sam L.
Applied Biosystems Inc.
Krupen Karen I.
Macevicz Steven C.
Nucker Christine M.
Smith Joseph H.
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