Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2003-09-19
2010-10-19
Babic, Christopher M. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
07816077
ABSTRACT:
It is intended to provide a non-invasive and convenient method of detecting a tumor marker for diagnosing colon cancer which is superior in sensitivity and specificity to the existing fecal occult blood test. More specifically speaking, a method of detecting a tumor marker for diagnosing colon cancer which comprises collecting biological sample which is immediately frozen using liquid nitrogen in some cases, homogenizing the sample in the presence of an inhibitor of an RNA digesting enzyme to give a suspension, extracting RNA from the obtained suspension, subjecting the extracted RNA to reverse transcription to give cDNA, amplifying the obtained cDNA and then detecting the thus amplified cDNA. This method is characterized by involving no procedure of separating cell components from the biological sample.
REFERENCES:
patent: 4843155 (1989-06-01), Chomczynski
patent: 5654179 (1997-08-01), Lin
patent: 5777099 (1998-07-01), Mehra
patent: 6258541 (2001-07-01), Chapkin et al.
patent: 6329179 (2001-12-01), Kopreski
patent: 6406857 (2002-06-01), Shuber et al.
patent: 7101663 (2006-09-01), Godfrey et al.
patent: 0 389 063 (1990-09-01), None
patent: 1 674 869 (2006-06-01), None
patent: 2002-142767 (2002-05-01), None
patent: WO 00/63358 (2000-10-01), None
patent: WO 2005/019827 (2005-03-01), None
patent: WO 2007/018257 (2007-02-01), None
Alexander and Raicht (1998) Digestive Diseases and Sciences vol. 43 No. 12 pp. 2652-2658.
Biotecx Bulletin No. 28, 1993.
Sano et al. (1995) Cancer Research 55: 3785-3789.
Lagerholm et al. (Apr. 2001) Gastroenterology vol. 120 No. 5 supplement 1 Abstract No. 16 in p. A4.
Ambion Poly A+ RNA Kit 2000.
Murphy et al. entitled “Gene expression studies using microarrays: principles, problems, and prospects,” (Adv Physiol Educ. Dec. 2002;26(1-4):256-70).
Lucentini, who entitled an article, “Gene Association Studies Typically Wrong” (The Scientist, 18(24):20).
Grigoryev et al. (Genome Biol. 2004;5(5):R34. Epub Apr. 27, 2004).
D. Sidransky et al., “Identification ofrasOncogene Mutations in the Stool of Patients with Curable Colorectal Tumors,”Science, 256, Apr. 3, 1992, 102-105.
S.M. Dong et al., “Detecting Colorectal Cancer in Stool With the Use of Multiple Genetic Targets,”Journal of the National Cancer Institute, 93 (11), Jun. 6, 2001, 858-865.
G. Traverso et al., “Detection ofAPCMutations in Fecal DNA From Patients With Colorectal Tumors,”The New England Journal of Medicine, 346 (5), Jan. 31, 2002, 311-320.
G. Traverso et al., “Detection of Proximal Colorectal Cancers Through Analysis of Faecal DNA,”The Lancet, 359, Feb. 2, 2002, 403-404.
Fang Sheng, Wu Pei, Liu Yongzhong, et al., “Clinical Investigation of Expression of Gastrin mRNA in Colorectal Carcinoma,”Proceedings of Wannan Medical College, vol. 21, (2002), 20-23.
Hiroki Nakagawa, “Bio Jikken Illustrated 2 Idenshi Kaiseki no. Kiso”, Shujunsha Co., Ltd., Sep. 25, 1995, pp. 153 to 166.
L.A. Davidson et al., “Non-invasive detection of fecal protein kinase C βIIand ç messenger RNA: putative biomarkers for colon cancer,” Carcinogenesis, vol. 19, No. 2, (1998), pp. 253 to 257.
T. Yamao et al., “Abnormal Expression of CD44 Variants in the Exfoliated Cells in the Feces of Patients With Colorectal Cancer,” Gastroenterology, vol. 114, No. 6, (1998), pp. 1196 to 1205.
R.L. Alexander et al., “Purification of Total RNA from Human Stool Samples,” Digestive Diseases and Sciences, vol. 43, No. 12, (1998), pp. 2652 to 2658.
Hajime Sano et al., “Expression of Cyclooxygenase-1 and -2 in Human Colorectal Cancer,” Cancer Research, vol. 55, No. 17, (1995), pp. 3785 to 3789.
S. Kanaoka et al., “Shou P-379 Potential of colorectal cancer screening by using RT-PCR method in which COX-2 from feces is used as a marker,” Japanese Journal of Gastroenterology, vol. 99, special extra issue, Sep. 20, 2002, p. A634 Sho P-379.
Rainer Deuter et al., “A method for preparation of fecal DNA suitable for PCR,”Nucleic Acids Research, 1995, vol. 23, No. 18, pp. 3800 to 3801.
Kanaoka S. et al., “Potential Usefulness of Detecting cyclooxygenase 2 Messenger RNA in Feces for Colorectal Cancer Screening,”Gastroenterology, (2004), vol. 147, No. 2, pp. 422-427.
Nosho K. et al., “Association of Ets-related transcriptional factor E1AF expression with overexpression of matrix metalloproteinases, COX-2 and iNOS in the early stage of colorectal carcinogenesis,”Carcinogenesis, (2005), vol. 26, No. 5, pp. 892-899.
Boedefeld W.M. et al., “Increased expression of E1A-F, MMP-7, and COX-2 in human colorectal cancer,”Proceedings of the American Association for Cancer Research Annual Meeting, (2002), vol. 43, p. 730, #3623.
Li X. et al., “Co-Expression of Matrix Metalloproteinase-7 and Cyclooxygenase-2 in Human Colorectal Carcinoma ”Proceedings of the American Association for Cancer Research Annual Meeting, (2002), vol. 42 , p. 614, #3302.
De Craene B. et al., “The Transcription Factor Snail Induces Tumor Cell Invasion through Modulation of the Epithelial Cell Differentiation Program,”Cancer Research, (2005), vol. 65, No. 14, pp. 6237-6244.
Roy H.K. et al., “The Transcriptional Repressor SNAIL Is Overexpressed in Human Colon Cancer,”Digestive Diseases and Sciences, (2005), vol. 50, No. 1, pp. 42-46.
Dutta S.K. et al., “Noninvasive Detection of Colorectal Cancer by Molecular tools: Coming of Age,”Gastroenterology, (1998), vol. 114, No. 6, pp. 1333-1335.
Kanemoto S. et al., “Funbenchu no COX-2 mRNA o Hyoteki ni shita DaichoganShindan Kenshin no Kanosei ni Tsuite,”Nippon Shokaki Shudan Kenshin Gakkai Zasshi, (2004), vol. 42, No. 2, p. 58 (S3-3).
Shattuck-Brandt, R.L. et al., “Differential Expression of Matrilysin and Cyclooxygenase-2- in Intestinal and Colorectal Neoplasms,”Molecular Carcinogenesis, 1999, vol. 24, No. 3, 1999, pp. 177-187.
Kanaoka Shigeru et al., “RNA-based stool assay is superior to a single immunochemical fecal occult blood test for detecting early colorectal cancer and adenoma,”Gastroenterology, vol. 130, No. 4, Suppl. 2, Apr. 2006, p. A471, Digestive Disease Week Meeting/107th Annual Meeting of the American-Gastroenterological-Association; Los Angeles, CA, USA, May 19-24, 2006.
Takai Tetsunari et al., “Potential usefulness of detecting cyclooxygenase 2 and matrix metalloproteinase 7 messenger RNA in feces for colorectal cancer screening,”Gastroenterology, vol. 130, No. 4, Suppl. 2, Apr. 2006, p. A188, Digestive Disease Week Meeting/107th Annual Meeting of the American-Gastroenterological-Association, Los Angeles, CA, USA, May 19-24, 2006.
Kanaoka Shigeru et al., “A comparison of fecal RNA test with immunochemical fecal occult blood test for detecting colorectal cancer and adenoma,”Gastroenterology, vol. 132, No. 4, Suppl. 2, Apr. 2007, p. A623, Digestive Week Meeting/108th Annual Meeting of the American-Gastroenterological-Association, Washington, DC., USA, May 19-24, 2007.
Kanaoka Shigeru et al., Potential usefulness of predicting stage of colorectal cancer by fecal RNA test,Gastroenterology, vol. 134, No. 4, Suppl. 1, Apr. 2008, p. A183, Digestive Disease Week Meeting/109th Annual Meeting of the American-Gastroenterological-Association, San Diego, CA, USA, May 17-22, 2008.
European Examination Report dated Mar. 5, 2010, issued for corresponding European Patent Application 06 782 598.4.
Ioannidis, John P.A. et al.; “Replication validity of genetic association studies”; Nature Genetics, vol. 29, Nov. 2001, p. 306-309.
Leung, Wai K. et al.; “Detection of Hypermethylated DNA or Cyclooxygenase-2 Messenger RNA in Fecal Samples of Patients With Colorectal Cancer of Polyps”; The American Journal of Gastroenterology, vol. 102, May 2007, p. 1070-1076 (Abstract Only).
Wacholder, Sholom et al.; “Assessing the Probability That a Positive Report is False:
Babic Christopher M.
Hamamatsu Foundation for Science and Technology Promotion
Olympus Corporation
Westerman Hattori Daniels & Adrian LLP
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