Method of detecting bovine diarrhoea virus infection, nucleotide

Details Method of detecting bovine diarrhoea virus infection, nucleotide Method of detecting bovine diarrhoea virus infection, nucleotide

1995-04-05

1997-09-09

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, C12Q 170, G01N 3353

Patent

active

056655347

ABSTRACT:
The invention is directed to a method of detecting infection of a blood sample by various strains of bovine diarrhoea virus (BVD) including a first test wherein anti-bovine diarrhoea virus antibodies are detected by means of a recombinant antigen comprising the BVD p80 protein expressed by a eukaryotic host and a second test wherein viral particles are detected by means of antibodies directed against the BVD p80 viral protein. The method is particularly useful in detecting pathogenic conditions such as persistent viremias and acute infections caused by BVD viruses, and is particularly advantageous because it is an extremely sensitive and efficient test and because it is capable of detecting infections caused by any one of a wide variety of BVD strains. The recombinant antigen employed in the method is preferably encoded by the nucleotide sequence listed as SEQ ID No: 1.

REFERENCES:
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Veterinary Microbiology, vol. 23, 1990, Amsterdam NL pp. 193-201; C. LeComte et al., `ELISA detection of bovine viral diarrhoea virus specific antibodies. . . `.
Virology, vol. 184, No. 1, 1988, pp. 191-199; M.S. Collett "Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus".
Biochemical and Biophysical Research Communications, vol. 178 No. 3, 15 Aug. 1991, pp. 1326-1334; J. Kwang et al, "Expression of the p80 region of bovine viral diarrhea . . . ".
Tiollais et al, Nature 317, 489-495 (1985) "hepatitis B Virus".
Atkinson et al, Pestic Sci 28(2):215-224 (1990) "Baculovuruses as Vectors for foreign gene expression . . . " (Biosis Abstract).

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