Method of detecting anti-leishmania parasite antibodies

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 795, 435975, 530324, 530822, 530810, G01N 33569

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active

054118654

ABSTRACT:
A method for detecting anti-Leishmania parasite antibodies to a 230 kDa antigen present in Leishmania chagasi and Leishmania donovani is disclosed which comprises obtaining a sample from an individual, contacting the sample with a recombinant K39 repeat unit antigen comprising the amino acid sequence as shown in SEQ ID NO:3, and detecting the presence of anti-Leishmania parasite antibodies in the sample which bind to the recombinant K39 repeat unit antigen.

REFERENCES:
Burns et al, "Molecular Characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American viscerol leishmaniasis," Proc. Natl. Acad. Sci. USA, 90(2): 775-779 (Jan. 15, 1993).
Zhang et al, "Use of a Recombinant 170-Kilodalton Surface Antigen of Entamoeba histolytica for Serodiagnosis of Amebiasis and Identification of Immunodominant Domains of the Native Molecule", J. Clin. Microbiol., 30(11):2788-2792 (Nov. 1992).

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