Method of detecting and using an expression-enhancing sequence

Details Method of detecting and using an expression-enhancing sequence Method of detecting and using an expression-enhancing sequence

2004-10-25

2008-12-23

Qian, Celine (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

C435S006120, C435S029000

Reexamination Certificate

active

07468260

ABSTRACT:
The invention relates to a method of detecting a DNA sequence which at least partially contributes to promote the stable expression of a gene. To this end the DNA fragment to be examined is cloned in a vector between i) a DNA sequence involved in the induction of gene transcription repressing chromatin and ii) a reporter gene. The invention also relates to the detected DNA sequence, and the application of a stable expression-enhancing DNA sequence for the stable expression of a gene.

REFERENCES:
patent: 5721096 (1998-02-01), Karathanasis et al.
patent: WO 94 29468 (1994-12-01), None
patent: WO 97 10337 (1997-03-01), None
patent: WO 98 11207 (1998-03-01), None
Mallin et al. Polycomb group repression is blocked by the Drosophila suppressor of Hairy-wing [su(Hw)] insulator. Genetics, vol. 148, pp. 331-339, Jan. 1998.
Greene et al. Subcloning. Methods in Enzymology, vol. 152, pp. 512-522, 1987.

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