Method of detecting and quantifying ligands in liquids via bioti

Details Method of detecting and quantifying ligands in liquids via bioti Method of detecting and quantifying ligands in liquids via bioti

1987-02-11

1989-09-05

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436501, 436518, 436536, 436541, 436543, 436546, 436800, 436822, G01N 33566, G01N 33543, G01N 33542, G01N 33533

Patent

active

048638765

ABSTRACT:
A method of determining a ligand of interest in a ligand is described. The method, which makes it possible to detect and quantify a ligand in a liquid, makes use of a biotin-avidin system which can be used to carry out immunoassays in both heterogeneous and homogeneous format. The basic components used in the method are a biotin-labeled substance (which is biotin-lableled ligand or biotin-labeled specific binding partner), biotin-labeled fluorophore, a liquid to be analyzed for the ligand of interest, specific binding partner for the ligand of interest and avidin.

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