Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-11-20
2000-08-22
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
5303871, 5303877, 435 6, G01N 3353, C07K 1600, C12Q 168
Patent
active
061070483
ABSTRACT:
A method of detecting dysplastic regions within epithelial tissue samples is sensitive enough to detect and distinguish between low grade and high grade dysplastic regions. The method uses probes specific for expression and accumulation of substances within a particular intracellular region from a defect in apical membrane trafficking (trafficking markers) and in the preferred embodiment correlates the trafficking marker levels with the presence of an oncogene such as p53. If low grade dysplasia is present, trafficking markers are detected in a distinctive perinuclear pattern. Previous studies have demonstrated a high correlation of p53 over-expression with high grade dysplasia and adenocarcinoma. Detection of p53 is shown to be mutually exclusive of detection of trafficking markers. Therefore, dual detection for both the trafficking markers and p53 provides an accurate method for more precise grading of biopsies. This definitive assay and grading assists in identifying populations at risk for progression to adenocarcinoma. Preferred trafficking markers include Rab11, Rab25, g-adaptin and mannose-6-phosphate receptor.
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Goldenring James R.
Lee Jeffrey R.
Ray Gregory S.
Guzo David
Medical College of Georgia Research Institute Inc.
Shuman Jon
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