Method of detecting and counting bacteria

Details Method of detecting and counting bacteria Method of detecting and counting bacteria

1975-03-05

1976-07-27

Tanenholtz, Alvin E.

Textiles: fiber preparation

C12K 100

Patent

active

039717038

ABSTRACT:
An improved method is provided for determining bacterial levels, especially in samples of aqueous physiological fluids, which method depends on the quantitative determination of bacterial adenosine triphosphate (ATP) in the presence of non-bacterial ATP. The bacterial ATP is released by cell rupture and is measured by an enzymatic bioluminescent assay. A concentration technique is included to make the method more sensitive. It is particularly useful where the fluid to be measured contains an unknown or low bacteria count.

REFERENCES:
patent: 3616253 (1971-10-01), D'Eustachio
patent: 3745090 (1973-07-01), Chappelle et al.

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