Method of detecting and characterizing a nucleic acid or reactan

Details Method of detecting and characterizing a nucleic acid or reactan Method of detecting and characterizing a nucleic acid or reactan

1982-04-29

1986-04-08

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435188, 435 18, 435810, C12Q 168, C12Q 134, G01N 3354, C12N 996

Patent

active

045813337

ABSTRACT:
Method for detecting the possible presence of a DNA fragment, notably of a gene, in the midst of a complex sample of nucleic acids.
It comprises the hybridization of the sought fragment with a RNA probe, this being, prior or subsequent to the hybridization reaction, modified by an enzyme.
Application to seeking of particular genes or DNA fragments in the midst of a biological sample.

REFERENCES:
patent: 4002532 (1977-01-01), Weltman et al.
patent: 4067774 (1978-01-01), Rubenstein et al.
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4318980 (1982-03-01), Boguslaski et al.
Manning et al., Biochemistry, 16(7):1364-1370 (1977).
Noyes et al., Cell, 5:301-310 (1975).
Reiser et al., Biochem. Biophys. Res. Comm., 85(3):1104-1112 (1978).
Wetmur, Biopolymers, 14:2517-2524 (1975).
Manning et al., Chromosoma (Berl.), 53:107-117 (1975).

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