Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-06-05
1997-12-02
Allen, Marianne P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 79, 435 792, 435 794, 435 795, 436518, G01N 3353
Patent
active
056934785
ABSTRACT:
This disclosure concerns a method of detecting the presence of a polypeptide in a sample wherein the polypeptide is encoded by a fragment of a nucleic acid molecule encoding an amyloid precursor mutein and the fragment comprises a sequence encoding at least one marker and a sequence encoding about 419 amino acid residues of the APP-695 isoform, about 475 amino acid residues of the APP-751 isoform or about 494 amino acid residues of the APP-770 isoform. The method encompasses contacting the sample with an antibody, which specifically binds the marker or the amyloid precursor mutein, under suitable conditions to favor the formation of an antibody-antigen complex and detecting the presence of any complex thus formed. The disclosure also deals with the method employing the above nucleic acid fragment wherein the amino acid residues from position 11 to position 28 are deleted from the portion of the sequence encoding the .beta.-amyloid protein domain and in which an alanine substitution at a phosphorylation site within the cytoplasmic domain of the amyloid precursor protein is encoded.
REFERENCES:
Vitek et al, Society for Neuroscience Abstracts, vol. 17, p. 1443, Abstract 572.3, 1991.
Jacobsen Jack Steven
Vitek Michael Peter
Allen Marianne P.
American Cyanamid Company
Barnhard Elizabeth M.
Duffy Patricia A.
Rosenblum Anne M.
LandOfFree
Method of detecting amyloid precursor proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of detecting amyloid precursor proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting amyloid precursor proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-800299