Method of detecting amplified nucleic sequences in cells by flow

Details Method of detecting amplified nucleic sequences in cells by flow Method of detecting amplified nucleic sequences in cells by flow

1994-05-18

1998-11-24

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

135 6, 135 912, 935 78, C12Q 170, C12Q 168, C12P 1934

Patent

active

058404783

ABSTRACT:
The field of this invention is the detection and isolation of specific amplified DNA sequences by flow cytometry. More particularly, this invention relates to the detection of these specific amplified DNA sequences in cells so as to allow quantitation of viral burden of patients infected with a virus. The method is particularly adapted to detection of HIV-1 proviral DNA sequences and the assessment of activity of the virus in a cell.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4770992 (1988-09-01), Van den Engh et al.
patent: 5436144 (1995-07-01), Stewart et al.
patent: 5480783 (1996-01-01), Haff
Komminoth, et al. (1992) Diagnostic Molecular Biology 1:85-87 Jun.
Timm, E.A, et al. Genotyping Cells by Flow Cytometry Using PCR Techniques. Abstracts for the XIV International Meeting of the Society for Analytical Cytology, Mar. 18-23, 1990. (Abstract 481B-p.79).
Costa Taveira, N., et al. Detection of HIV1 proviral DNA by PCR and hybridization with digoxigenin labelled probes. Int. Con. AIDS (Jun 20, 199)6:157 (Abstract No. F.A. 327).
Seibl, R., et al Non-Radioactive Labelling and Detection of Nucleic Acids. III. Applications of the Digoxigenin System. Biol. Chem. Hoppe-Seyler (Oct. 1990) 371:939-951.
Meyer et al. DNA Flow Cytometry of Breast Carcinoma After Acetic-Acid Fixation, Cell. Tissue Kinet. (1984) 17:184-197. (Abstract).
Tournier et al. Detection of Albumith mRNAs in Rat Liver by Insitu Hybridization: Usefulness of Paraffin Embedding and Comparison of Various Fixation Procedures. J.Histochem Cytochem (Apr. 1987) 35:453-459 (Abstract).
P. H. Hinderling, Kinetics of Partitioning and Binding of Digoxin and Its Analogues in the Sub Compartments of Blood. J.Pharm Sci (1984)73:1042-1052.
Basgasra et al., Detection of Human Immunodeficiency Virus Type I Provirus in Monocuclear Cells by In Situ Polymerase Chain Reaction, N. Eng. J. Med. 326, 1385 (1992).
Bauman et al., Flow Cytometric Detection of Ribosomal RNA in Suspended Cells by Fluorescent In Situ Hybridization.sup.1,2, Cytometry 9:515-524 (1988).
Dudin et al., A Method for Nucei Acid Hybridization to Isolated Chromosomes in Suspension, Hum. Genet 76:190-292 (1987).
Folks et al., Biological and Biochemical Characterization of a Cloned LEU-3 Cell Surviving Infection with the Acquired Immune Deficiency Syndrome Retrovirus, J. Exp. Med. 164:280-290 (1986).
Harper et al., Detection of Lymphocytes Expressing Human T-lymphotropic virus Type III in Lymph Nodes and Peripheral Blood from Infected Individuals by in situ hybridization, Proc. Natl. Acad. Sci USA 83;772-776 (1986).
Hsia et al., Human Immunodeficiency virus DNA is Present in a High Percentage of CD4.sup.30 Lymphocytes of Seropositive Individuals, J. Infect. Dis. 164:470-475 (1991). Polymerase-Catalyzed Chain Reaction, Methods of Enzymo. 155:335-350 (1987).
Ou et al., DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear Cells, Science 238:295-297 (1988).
Pinkel et al., Cytogenetic Analysis by In Situ Hybridization with Fluorescently Labeled Nucleic Acid Probes, Cold Spring Harbor Symposia on Quantative Bio. LI:151-157 (1986).
Saiki et al., Enzymatic Amplification of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia, Science 230:1350-1354 (1985).
Saiki et al., Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase, Science 239:487-491 (1988).
Schnittman et al., The Reservoir for HIV-1 in Human Peripheral Blood Is a T Cell That Maintains Expression of CD4, Science 245:305-308 (1989).
Schnittman et al., Increasing Viral Burden in CD4+ T Cells from Patients with Human Immunodeficiency Virus (HIV) Infection Reflects Rapidly Progressive Immunosuppression and Clinical Disease, Ann. Intern. Med. 113:438-443 (1990).

Affiliated with

Also associated with

Rating

Method of detecting amplified nucleic sequences in cells by flow does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of detecting amplified nucleic sequences in cells by flow, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of detecting amplified nucleic sequences in cells by flow will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1700524