Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1986-05-06
1990-04-17
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
422 61, 424 3, 424 71, 435 7, 435 29, 435 32, 435 68, 43524027, 435810, 530387, 935102, 935103, 935104, 935105, 935106, 935108, 935110, G01N 33569, G01N 33577
Patent
active
049179985
ABSTRACT:
A method is disclosed for detecting the presence of HTLV III infected cells in a medium. The method comprises contacting the medium with monoclonal antibodies against an antigen produced as a result of the infection and detecting the binding of the antibodies to the antigen. The antigen may be a gene product of the HTLV III virus or may be bound to such gene product. On the other hand the antigen may not be a viral gene product by may be produced as a result of the infection and may further be bound to a lymphocyte. The medium may be a human body fluid or a culture medium. A particular embodiment of the present method involves a method for determining the presence of an AIDS virus in a person. The method comprises combining a sample of a body fluid from the person with a monoclonal antibody that binds to an antigen produced as a result of the infection and detecting the binding of the monoclonal antibody to the antigen. The presence of the binding indicates the presence of an AIDS virus infection. Also disclosed are novel monoclonal antibodies, novel compositions of matter, and novel diagnostic kits.
REFERENCES:
patent: 4629783 (1986-12-01), Cosand
McDougal et al, "Immunoassay for the Detection and Quantitation of Infectious Human Retrovirus, Lymphadenopathy-Associated Virus (LAV)", Journal of Immunological Methods, 76(1985):171-183.
Gallo et al, "Isolation of Human T-Cell Leukemia Virus in Acquired Immune Deficiency Syndrome (AIDS)", Science, 220(1983):865-867.
Robert-Guroff et al, "A Monoclonal Antibody Specific for a 52,000-Molecular-Weight Human T-Cell Leukemia Virus-Associated Glycoprotein Expressed by Infected Cells", Journal of Virology, 53(1985):214-220.
Hoxie et al, "Persistent Noncytopathic Infection of Normal Human T Lymphocytes with AIDS-Associated Retrovirus", Science 229:1400-1402 (Sep. 27, 1985).
Sarngadharan et al, "Immunological Properties of HTLV-III Antigens Recognized by Sera of Patients with AIDS and AIDS-related Complex and of Asymptomatic Carriers of HTLV-III Infection", Cancer Research 45:4574s-4577s (Sep. 1985).
Barre-Sinoussi et al, "Analysis and Immunological Properties of Lymphadenopathy Associated Virus (LAV) Structural Proteins", from: International Symposium: Retroviruses and Human Pathology, Humana Press, Clifton, N.J. (1985), 343-351.
Fauci, "Immunopathogenesis of the Acquired Immune Deficiency Syndrome", from: Vaccines 86:New Approaches to Immunization, Cold Spring Harbor Laboratory, New York (1986), 323-325.
Montagnier et al, "Identification and Antigenticity of the Major Envelope Glycoprotein of Lyphadenopathy-Associated Virus", Virology 144:283-289 (1985).
Sayers et al, "HLA Antibodies as a Cause of False Positive Reactions in Screening Enzyme Immunoassays for Antibodies to Human T-Lymphotropic Virus Type III", Transfusion 26:113-115 (1986).
McDougal et al, "Cellular Tropism of the Human Retrovirus HTLV-III/LAV:I.Role of the T Cell Activation and Expression of the T4 Antigen", J. Immunol. 135:3151-3162 (Nov. 1985).
McDougal et al, "Binding of HTLV-III/LAV to T4.sup.+ T Cells by a Complex of the 110K Viral Protein and the T4 Molecule", Science 231:382-385 (Jan. 24, 1986).
Auffray et al, "Speculations on Sequence Homologies Between the Fibronectin Cell-Attachment Site, Major Histocompatability Antigens, and a Putative AIDS Virus Polypeptide", Human Immunol. 15:381-390 (1986).
Sandstrom et al, "Detection of Human Anti-HTLV-III Antibodies by Indirect Immunofluorescence Using Fixed Cells", Transfusion 25:308-12 (1985).
Sarin, et al., Biochemical Pharmacology, vol. 34, No. 22, pp. 4075-4079, 1985 "Inhibition of HTLV-III/LAV Replication by Foscarnet".
Burger Denis R.
Goldstein Andrew S.
Cooper Iver P.
Epitope, Inc.
Nucker Christine M.
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