Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Patent
1986-09-10
1989-08-22
Griffin, Ronald W.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
514 57, 514 60, 514835, 424 49, 523105, A61K 31715, A61K 600, A61K 31725, A61K 3306
Patent
active
048596566
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a method of greatly increasing the detachment of micro-flora adhered to various types of boundary surfaces/inter-phases by the addition of various combinations of polymers, preferably biopolymers, to treatment solutions. The invention also relates to preparations for achieving the above-mentioned result.
In our earlier patent application (Pat. app. No. 8206250-6) we were able to establish that bacteria irreversibly adhered to various boundary surfaces can be made to detach themselves to a greater or lesser extent simply due to the action of concentrated salt solutions.
This is illustrated by, but not limited to the following experiments in a model system.
A number of glass tubes, all the same size, were immersed in a suspension of bacteria marked with a radioactive isotope (tritium) and the bacteria were allowed to adhere over a period of 30 minutes. The glass tubes were then removed one by one and rinsed by repeated dipping (30 times) in a buffer solution in order to remove reversibly bound bacteria. Only the irreversibly bound bacteria then remained on the glass tube. The glass tube was then immersed in a salt solution for 5 minutes. After rinsing, the quantity of bacteria adhered to the tube was determined by measuring the radiation in a liquid scintillator.
A comparison of the various glass tubes which had been immersed in salt solution of various concentrations, with control tubes which had undergone the same treatment with the exception of immersion in the salt solution, enabled assessment of the effect of the salt solution on the irreversibly bound bacteria. The table below and the accompanying figures show the effects of the various salts on detachment of the irreversibly adhered bacteria, expressed as a percentage of the quantity of irreversibly adhered bacteria before the salt treatment. The concentrations of the solutions are in multiples of a specific critical concentration (C) of the basic solution.
Of the salts tested in this series of experiments, magnesium sulphate (MgSO.sub.4) gave the strongest detaching effect. However, magnesium chloride (MgCl.sub.2) and ammonium acetate (NH.sub.4 Ac) solutions also gave good results, whereas ammonium sulphate ((NH.sub.4).sub.2 SO.sub.4) solutions gave a slightly different pattern.
Several other compounds and salts have been tested besides those mentioned in this series of experiments, and the results were similar.
TABLE
Detachment percentage of irreversibly adhered bacteria after treatment.
The number of bacteria irreversibly adhered to the glass tube was 3.0.times.10.sup.6 before treatment.
Salt concentration in multiples of basic solution C
______________________________________ Salts 0.05 .times. C
0.1 .times. C
0.2 .times. C
0.5 .times. C
______________________________________
MgSO.sub.4
41 54 28 28
MgCl.sub.2
20 40 21 25
NH.sub.4 Acetate
8.5 41 17 24
(NH.sub.4).sub.2 SO.sub.4
31 36 44 28
______________________________________
The detachment of microorganisms as a result of exposure to solutions of salts and/or polymers can be seen in the attached drawings, in which:
FIG. 1 shows the detachment as a percentage of Streptococcus mutans bacteria bound irreversibly to glass, in relation to several salts at various concentrations.
FIG. 2 shows detachment as a percentage of Streptococcus mutans bacteria bound irreversibly to hydroxyl apatite, in relation to magnesium sulfate at various concentrations.
FIG. 3 shows detachment as a percentage of Streptococcus mutans bacteria bound irreversibly to glass, in relation to a combination of magnesium sulfate at various concentrations, with various polymers at 1% by weight.
FIG. 4 shows detachment as a percentage of Streptococcus mutans bound irreversibly to glass, in relation to magnesium sulfate at various concentrations, together with combinations of two polymers.
FIG. 1 shows the detachment of irreversibly adhered bacteria after treatment with salt solutions of various concentrations. The salt concentration is expressed in mult
REFERENCES:
patent: 4282204 (1981-08-01), Phillips et al.
patent: 4327079 (1982-04-01), Aoki
patent: 4360513 (1982-11-01), Buck
patent: 4537767 (1985-08-01), Rothman et al.
patent: 4619825 (1986-10-01), Eigen et al.
patent: 4704360 (1987-11-01), Shoham et al.
Kjelleberg Staffan
Ronnow Peter
Carson Nancy S.
Chemical Dynamics Sweden AB
Griffin Ronald W.
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