Method of desulfurization of fossil fuel with flavoprotein

Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Petroleum oil or shale oil treating

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435189, 435281, C12N 902, C10G 3200

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active

059856505

ABSTRACT:
The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of a flavoprotein to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of:

REFERENCES:
patent: 5356801 (1994-10-01), Rambosek et al.
patent: 5733773 (1998-03-01), Squires et al.
Denome, Sylvia A. et al., "Characterization of the Desulfurization Genes from Rhodococcus sp. Strain IGTS8," Journal of Bacteriology 176 (21) :6707-6716 (1994).
Lei, Benfang et al., "Vibrio harveyi NADPH-Flavin Oxidoreductase: Cloning, Sequencing and Overexpression of the Gene and Purification and Characterization of the Cloned Enzyme," Journal of Bacteriology 176 (12):3552-3558 (1994).
Ohshiro, Takashi et al., "Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erthropolis D-1," FEMS Microbiology letters 118:341-344 (1994).
Ohshiro, Takashi et al., "Involvement of Flavin Coenzyme in Dibenzothiophene Degrading Enzyme System from Rhodococcus erythropolis D-1," Biosci. Biotech. Biochem., 59(7): 1349-1351 (1995).
Piddington, Christopher S. et al., "Sequence and Molecular Characterization of DNA Region Encoding the Dibenzothiophen Desulfurization Operon of Rhodococcus sp. Strain IGTS8," Applied and Environmental Microbiology, 61 (2):468-475 (1995).
Nagy, Istvan et al., "Characterization of the Rhodococcus sp. NI86/21 gene encoding alcohol: N,N'-dimethyl-4-nitrosoaniline oxidoreductase inducible by atrazine and thiocarbamate herbicides," Arch Microbiol., 163:439-446 (1995).

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