Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2011-04-12
2011-04-12
Ton, Thaian N (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C435S366000, C435S368000
Reexamination Certificate
active
07923246
ABSTRACT:
The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.
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Kinoshita Shigeru
Sasai Yoshiki
Ueno Morio
Leydig , Voit & Mayer, Ltd.
Riken
Ton Thaian N
LandOfFree
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