Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1988-04-28
1990-11-27
Warden, Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352402, 435240241, 435291, 435313, 435818, C12N 500, C12M 104
Patent
active
049735586
ABSTRACT:
A method of culturing cells by providing a highly gassed media on a continuous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.
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Gaines, Jr. William A.
Harm William H.
Page Darrell P.
Wilson John R.
Endotronics, Inc.
Griffith Rebekah A.
Warden Robert J.
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