Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1979-11-06
1982-07-13
Naff, David M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435241, 435285, 435286, C12N 500, C12M 304
Patent
active
043395376
ABSTRACT:
Biological substances such as tissues, cells, vaccines and viruses are cultured by the steps of: introducing a culture solution having the substances suspended therein into a closed culture vessel having a plurality of disc-like implantation plates arranged horizontally with a certain space between successive plates so that a predetermined number of the implantation plates is immersed in the culture solution; culturing the substances on the surface of the implantation plates; removing the substances from the implantation plates; suspending the removed cultured substances in a new culture solution; and successively repeating the steps of culturing, removing the cultured substances and suspending in a new culture solution while each time increasing the amount of newly supplied culture solution to progressively increase the number of implantation plates immersed in the culture solution. This process enables production of a large amount of biological substance from a small amount of seed substance by mass production in a simple operation without any contamination.
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Hattori Shin-ichiro
Izawa Masao
Shinohara Daizo
Sogi Shinroku
Tachikawa Sachiko
Naff David M.
Olympus Optical Company Limited
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