Method of culturing anchorage dependent cells

Details Method of culturing anchorage dependent cells Method of culturing anchorage dependent cells

1983-04-13

1985-01-22

Naff, David M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435174, 435182, 435240, C12N 500, C12N 502, C12N 1100, C12N 1104

Patent

active

044952880

ABSTRACT:
Disclosed is a method of growing anchorage-dependent cells: cells of the type which normally undergo mitosis only when anchored on a substrate, e.g., fibroblasts or epithelial cells. The method comprises the steps of encapsulating a seed culture of the cells within a semipermeable membrane and suspending the capsules in a growth medium. The interior surfaces of the capsule membrane and/or collagen enclosed within the capsules serve as a substrate for the cells. The ratio of the available substrate surface area to the volume of the culture may be large, thereby allowing the cells to be grown substantially throughout the volume of the culture medium.

REFERENCES:
patent: 3730841 (1973-05-01), Forgione et al.
patent: 4189534 (1980-02-01), Levine et al.
patent: 4266032 (1981-05-01), Miller et al.
patent: 4352883 (1982-10-01), Lim

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