Method of cultivating cells of animal and human tissues

Details Method of cultivating cells of animal and human tissues Method of cultivating cells of animal and human tissues

1978-08-23

1981-03-31

Warden, Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

261 77, C12N 502

Patent

active

042594499

ABSTRACT:
A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas into the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.

REFERENCES:
patent: 2975065 (1961-03-01), White
patent: 3405920 (1968-10-01), Lefrancois
patent: 3506541 (1970-04-01), Snelling
patent: 4173516 (1979-11-01), Katinger et al.

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