Method of cultivating animal or plant cells

Details Method of cultivating animal or plant cells Method of cultivating animal or plant cells

1991-03-13

1993-01-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352402, 43524021, 43524023, 43524024, 435240242, 4352403, 43524031, 4352404, 43524045, 43524054, C12N 500, C12N 502, C12N 504, C12N 506

Patent

active

051806762

ABSTRACT:
A method of cultivating animal or plant cells, which consists essentially of contacting a cultivation liquor having animal or plant cells suspended therein with a liquid fluorocarbon having molecular oxygen dissolved therein, characterized in that said cultivation liquor forms a continuous suspension phase which has cell densities of at least 4.times.10.sup.6 cells/ml, that said contact is made by feeding said liquid fluorocarbon into the cultivation liquor from above the cultivation liquor such that 1 ml of liquid fluorocarbon has a surface area of about 6 to about 300 cm.sup.2 whereby the liquid fluorocarbon falls downward through the cultivation liquor by gravity, and that said cultivation liquor has a volume of at least about 10 liters.

REFERENCES:
patent: 3850753 (1974-11-01), Chibata et al.
patent: 3997396 (1976-12-01), Delente
patent: 4302905 (1981-12-01), Vasseen
Matthiason, et al. (1983) "Use of perfluorochemicals for oxygen supply to immobilized cells" Annals of the New York Academy of Sciences, USA vol. 413: 545-547.
Adlerereutz, et al. (1982) "Oxygen supply to immobilized cells" European Journal of Applied Microbiology vol. 16:165-170.
Damiano et al. (1985) "Normal use of a perfluorocarbon for supplying oxygen to aerobic submerged cultures" Biotechnology Letters, vol. 7:81-86.
Glacken et al. (1983) "Mammalian cell culture: engineering principles and scale-up" Trends in Biotechnology vol. 1:102-108.

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