Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1993-02-16
1994-11-15
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
4352351, 4352402, 435260, 62 62, A01N 102, C12N 700, C12N 500, C12N 104, F25D 2500
Patent
active
053647564
ABSTRACT:
This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.
REFERENCES:
patent: 3228838 (1966-01-01), Rinfret
patent: 4205132 (1980-05-01), Sandine et al.
patent: 4229544 (1980-10-01), Haynes et al.
patent: 4329787 (1982-05-01), Newton
patent: 4559298 (1985-12-01), Fahy
patent: 4567847 (1986-02-01), Linner
patent: 4688387 (1987-08-01), Conaway
patent: 4707998 (1987-11-01), Linner et al.
patent: 4807442 (1989-02-01), Linner et al.
patent: 4865871 (1989-09-01), Livesey et al.
patent: 4874690 (1989-10-01), Goodrich, Jr.
patent: 4964280 (1990-10-01), Piunno et al.
patent: 4980277 (1990-12-01), Junnilla
patent: 5024830 (1991-06-01), Linner et al.
patent: 5045446 (1991-09-01), Goodrich, Jr.
patent: 5153004 (1992-10-01), Goodrich, Jr.
patent: 5171661 (1992-12-01), Goodrich, Jr.
patent: 5178884 (1993-01-01), Goodrich
Lejeune et al., Ann. Pharm. Fr., 44(6), pp. 461-466 (1986).
Hayakawa, K., Hakkokogaku Kaisha, 63 (1) pp. 17-22 (1985).
Roinel, N., J. Electron. Microsc. Tech., 9(1) pp. 45-46 May 1988.
Meryman, H. T., "Absence of Unfrozen Freezable Water in Rapidly Frozen Red Cells," 7 Cryobiology, pp. 252-255 (1971).
Terracio, L. and Schwabe, K. G., "Freezing and Drying of Biological Tissues for Electron Microscopy," 29 Journal of Histochemistry and Cytochemistry, pp. 1021-1028 (1981).
Coulter, H. D. and Terracio, K., "Preparation of Biological Tissue for Electron Microscopy by Freeze-Drying," 187 Anatomical Record, pp. 477-493 (1977).
Escaig, Jacques, "New Instruments Which Facilitate Rapid Freezing at 83K and 6K," Journal of Microscopy, Jun. 1982, pp. 221-229.
Boyne, Alan F., "A Gentle, Bounce-Free Assembly for Quick-Freezing Tissues for Electron Microscopy: Application to Isolated Torpedine Ray Electrocyte Stacks," Journal of Neuroscience Methods, 1, pp. 353-364 (1979).
Coulder, H. David, "Freezing and Drying of Biological Tissues With a Toggle-Link Helium Freezer and an Improved Freeze-Drying Apparatus: Application to Neuropeptide Immunocytochemistry," Journal of Electron Microscopy Technique, 4, pp. 315-328 (1986).
Escaig, Jacques, "Control of Different Parameters for Optimal Freezing Conditions," Science of Biological Speciment Preparation, pp. 117-122.
Handley, Dean A., Alexander, Jack T., and Chien, Shu, "The Design and Use of a Simple Device for Rapid Quench-Freezing of Biological Samples," Journal of Microscopy, Mar. 1981, pp. 273-282.
Polaron Instruments, Inc., "The Slammer," (Brochure).
Med-Vac, Inc., "Cryopress" (Brochure).
Reichert-Jung, "Cryofrace 190 Cryoblock" (Brochure).
Quick Freezubg Devices, "Quick Freezing by Bounce-Free Delivery" (Brochure).
Moor, H., Bellin, G., Sandri, C. and Akert, K., "The Influence of High Pressure Freezing on Mammalian Nerve Tissue," Cell and Tissue Research, pp. 201-216 (1980).
Linner, et al. Chemical Abstracts, vol. 109, No. 15, Abstract No. 125237h, 1987.
Ootorii, Patent Abstracts of Japan, vol. 9, No. 21, Application No. 59-169504(A), 1985.
Moscow Meat Milk Ind., Derwent Abstract of SU395060, Acc. No. 42968V, 1974.
Mayer, "New method for vitrifying water and other liquids by rapid cooling of their aerosols" Jul. 15, 1985 J. Appl. Phys. 58(2) Jul. 1985 pp. 663-667.
del Campo Anthony A.
Livesey Stephen A.
Nag Abhijit
Dadio Susan M.
Lifecell
Robinson Douglas W.
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