Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor
Reexamination Certificate
2007-01-02
2007-01-02
Saucier, Sandra E (Department: 1651)
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including freezing; composition therefor
Reexamination Certificate
active
10646802
ABSTRACT:
A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than −15° C. to a cryoprotectant concentration sufficient for vitrification; cooling the tissue or organ at an average rate of from 2.5–100° C. per minute to a temperature between −80° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30° C. per minute to a temperature below the glass transition temperature to vitrify the tissue or organ. After the vitrified tissue or organ has been stored, the tissue or organ may be removed from vitrification by warming the tissue or organ at an average rate of from 20–40° C. per minute to a temperature between −80° C. and the glass transition temperature; further warming the tissue or organ at a rate greater than 80° C. per minute to a temperature above −75° C.; and reducing the concentration of the cryoprotectant. Tissues or organs treated in this manner exhibit near normal functions, for example, blood vessels exhibit near normal smooth muscle contractility and normal graft functions.
REFERENCES:
patent: 4494385 (1985-01-01), Kuraoka et al.
patent: 4559298 (1985-12-01), Fahy
patent: 5122110 (1992-06-01), McNally et al.
patent: 5145769 (1992-09-01), McNally et al.
patent: 5149621 (1992-09-01), McNally et al.
patent: 5158867 (1992-10-01), McNally et al.
patent: 5217860 (1993-06-01), Fahy et al.
patent: 5472876 (1995-12-01), Fahy
patent: 5518878 (1996-05-01), Wilkins et al.
patent: 5723282 (1998-03-01), Fahy et al.
patent: 5821045 (1998-10-01), Fahy et al.
patent: 5856081 (1999-01-01), Fahy
patent: 5873254 (1999-02-01), Arav
patent: 5962214 (1999-10-01), Fahy et al.
patent: 6194137 (2001-02-01), Khirabadi et al.
patent: WO 96/05727 (1996-02-01), None
patent: WO 00/60935 (2000-10-01), None
patent: WO 01/78504 (2001-10-01), None
Pegg et al., “Fractures in Cryopreserved Elastic Arteries”, Cryobiology 34 : 183-192 (1997).
Armitage, “Survival of Corneal Endothelium following Exposure to a Vitrification Solution,” CRYOBIOLOGY, vol. 26, 1989, pp. 318-327.
Bourne et al., “Human Corneal Studies with a Vitrification Solution Containing Dimethyl Sulfoxide, Formamide, and 1,2-Propanediol,” CRYOBIOLOGY, vol. 31, No. 6, 1994, pp. 522-530.
Dent et al., “Cryopreservation of Vein Grafts,” Surgical Forum, vol. 25, pp. 241-243, 1974.
Brockbank et al., “Cryopreserved Vein Transplantation,” Journal of Cardiac Surgery, vol. 7, No. 2, pp. 170-176, 1992.
Müller-Schweinitzer, “Cryopreservation: a useful technique for storing tissues for pharmacological investigations,” Trends in Pharmacological Sciences; vol. 9, No. 6, pp. 221-223; Jun. 1988.
Weber et al., “Viable Vein Graft Preservation,” Journal of Surgical Research 18, pp. 247-255, 1975.
Bishop et al., “A morphological assessment of vein allografts preserved in glycerol and used for arterial replacement,” Journal of Cardiovascular Surgery 28, pp. 491-497, 1987.
Barner et al., “Fresh and Frozen Homologous Venous Grafts for Arterial Repair,” Angiology 17, pp. 389-401, 1966.
Sitzmann et al., “Dimethylsulfoxide-treated, cryopreserved venous allografts in the arterial and venous systems,” Surgery, vol. 95, No. 2, pp. 154-159, 1984.
Song et al., “Cryopreservation of the Common Carotid Artery of the Rabbit: Optimization of Dimethyl Sulfoxide Concentration and Cooling Rate,” Cryobiology 32, pp. 405-421, 1995.
Wusterman et al., “The Effect of Cooling Rate and Temperature on the Toxicity of Ethylene Glycol in the Rabbit Internal Carotid Artery,” Cryobiology 33, pp. 423-429, 1996.
Narayanan et al., “Successful Cryopreservation of Microvenous Allografts,” Journal of Investigative Surgery, vol. 5, pp. 155-160, 1992.
Ku et al., “Human Coronary Vascular Smooth Muscle and Endothelium-Dependent Responses after Storage at -75° C,” Cryobiology 29, pp. 199-209, 1992.
Müller-Schweinitzer et al., “Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum,” Naunyn-Schmiedeberg's Archives of Pharmacology, pp. 1-4, 1992.
Müller-Schweinitzer et al., “Functional recovery of human mesenteric and coronary arteries after cryopreservation at—196° C in a serum-free medium,” Journal of Vascular Surgery, vol. 25, No. 4, pp. 743-749, 1997.
Fahy et al., G. M., “Vitrification as an Approach to Cryopreservation,” Cryobiology 21, pp. 407-426, 1984.
Chen et al., X. H., “Vitrification of Multicomponent Solutions by Cooling to Cryogenic Temperatures,” Cryogenics vol. 30 Sept. Supplement, pp. 541-545, 1990.
Fahy et al., G. M., “Some Emerging Principles Underlying the Physical Properties, Biological Actions, and Utility of Vitrification Solutions,” Cryobiology 24, pp. 196-213, 1987.
Ren et al., H. S., “The Crystallization Kimetics and the Critical Cooling Rates for Vitrification of Cryoprotective Solutions,” Cryogenics 1990, vol. 30, Sep. Supplement, pp. 536-540.
Guttman et al., Frank M., “Variation of Cooling Rate and Concentration of Dimethyl Sulfoxide on Rabbit Kidney Function,” Cryobiology, 23, pp. 495-499, 1986.
Jacobsen et al., I.A., “Effect of Cooling and Warming Rate on Glycerolized Rabbit Kidneys,” Cryobiology 19:668, 1982.
F. Binette et al., “Expression of a Stable Articular Cartilage Phenotype Without Evidence of Hypertrophy by Adult Human Articular Chondrocytes In Vitro”, Journal of Orthopaedic Research, vol. 16, pp. 207-216, 1998.
Y. Song et al., “Vitreous Cryopreservation Maintains the Function of Vascular Grafts”, Nature Biotechnology, vol. 18, pp. 296-299, 2000.
T. Fujita, MD, PhD, et al., “Successful Preservation of Human Skin by Vitrification”, Journal of Burn Care & Rehabilitation, vol. 21, No. 4, pp. 304-309, 2000.
B. Stone et al., “Cryopreservation of Human Articular Cartilage for Autologous Chondrocyte Implantation”, Cryobiology, (Abstracts, 35thAnnual Meeting), vol. 37, No. 4, pp. 445-446, 1998.
Abstracts, 36thAnnual Meeting of the Society for Cryobiology, “Vitrification of the Rabbit Cornea”, Cryobiology, vol. 38, pp. 310, 1999.
D.E. Pegg et al., “Fractures in Cryopreserved Elastic Arteries,” Abstract Only, Database Accession No. PREV199799558143, Cryobiology, vol. 34, No. 2, pp. 183-192, 1997.
Brockbank Kelvin G. M.
Khirabadi Bijan S.
Song Ying C.
Oliff & Berridg,e PLC
Organ Recovery Systems, Inc.
Saucier Sandra E
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