Method of cross-linking peptides

Details Method of cross-linking peptides Method of cross-linking peptides

2008-07-08

2008-07-08

Monshipouri, Maryam (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C530S350000, C435S183000, C435S193000

Reexamination Certificate

active

07396656

ABSTRACT:
A method of cross-linking a peptide to form a homopolymer of the peptide, to immobilize the peptide on a solid phase and to enhance antigenicity of the peptide is disclosed. The method comprises the steps of preparing a fusion peptide by incorporating a cross-linking segment including a tetrapeptide sequence Q-X-K-(S/T) (SEQ ID NO: 15) into the peptide and cross-linking the peptide by a glutaminase.

REFERENCES:
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patent: 5939385 (1999-08-01), Labroo et al.
S. E. Harris, et al., “Structural Characterization of the Rat Seminal Vesicle Secretion II Protein and Gene”, The Journal of Biological Chemistry, vol. 265, No. 17, pp. 9896-9903, 1990.
A. Lundwall et al., “Chemical Characterization of the Predominant Proteins Secreted by Mouse Seminal Vesicles”, Eur. J. Biochem, 249, pp. 39-44, (1997).

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