Method of covalently bonding biopolymer to a solid hydrophilic o

Details Method of covalently bonding biopolymer to a solid hydrophilic o Method of covalently bonding biopolymer to a solid hydrophilic o

1991-09-13

1993-03-30

Nutter, Nathan M.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525 542, 525 5421, 525 5423, 525 5424, 525 543, 525 5431, C08H 512, C08L 8900, C08L 100

Patent

active

051984938

ABSTRACT:
A process for covalently bonding biopolymer, such as protein, to an organic polymer surface coated with hydrophilic nonionic polymer having groups reactive with the biopolymer, which comprises reacting biopolymer with the surface in a nonpolar reaction medium, preferably a microemulsion, containing from about 0.5 to about 25% water by weight, having a dielectric constant less than 10% of the dielectric constant of pure water; and the product comprises a biopolymer immobilized on a hydrophilic solid surface having a nonionic polymer and a hydrophilic layer, coupled thereto via biopolymer-reactant groups of the nonionic polymer, and accordingly has low spontaneous adsorption of proteins and other biopolymers through electrostatic attraction and/or hydrophobic interaction.

REFERENCES:
patent: 4898824 (1990-02-01), Yip

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