Method of covalently binding biologically active organic substan

Details Method of covalently binding biologically active organic substan Method of covalently binding biologically active organic substan

1981-12-01

1983-11-15

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435178, 435180, C12N 1112, C12N 1110, C12N 1108

Patent

active

044156650

ABSTRACT:
The invention relates to a method of coupling organic ligands to a polymeric carrier. The organic ligand contains at least one primary or secondary amino group, at least one thiol group and/or at least one aromatic hydroxy group, and the polymeric carrier contains at least one hydroxy group. According to the invention the coupling is performed by forming a reactive derivative of the polymeric carrier by treatment with a sulfonyl halogenid, and reacting the formed reactive derivative with the organic ligand, which is thereby bonded directly to a carbon atom in the polymeric carrier. The polymeric carrier can, for example, be a possibly cross-linked polysaccharide, and the organic ligand is preferably a biological material such as a protein. The coupling product can, for example, be used for immunologic determinations, for affinity chromatography, etc. The coupling method can be performed under mild conditions not damaging sensitive organic ligands.

REFERENCES:
patent: 3959079 (1976-05-01), Mareschi et al.
patent: 3969287 (1976-07-01), Jaworek et al.
Weliky et al., The Chemistry and Use of Cellulose Derivatives for the Study of Biological Systems, Immunochemistry, vol. 2, 1965 (pp. 293-312).

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