Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-03-20
1994-04-12
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352402, 4351723, 935 11, 935 33, 935 34, C12P 2100, C12N 500, C12N 1500
Patent
active
053025172
ABSTRACT:
To control the production of a protein product by an animal cell culture, a culture of cells is grown to a density greater than one million cells per milliliter wherein the cells have first and second genes under the control of a single promoter with one of said first and second genes being expressed in an early protein which activates said promoter and the other being expressed in a late protein which is said protein product. A starting material for said culture is introduced into it, whereby said culture begins to replicate said early protein for activating said promoter in a self-stimulating manner to cause the other of said first and second genes express itself in said final product. The gene for the early proteins and the gene for the late proteins are artifically positioned into the same genomic region. The first and second promoters are incorporated in the same transactivation region. The vector material is taken from a parvovirus.
REFERENCES:
Tratschin et al., Mol. Cell. Biol. 6:2884-2894 (1986).
Rhode (a), J. Virology 55:886-889 (1985).
Gorman et al., Nucleic Acid Res. 11:7631-7648 (1983).
Sodioski et al., Science 227:171-173 (1985).
Gendelman et al., Proc. Natl. Acad. Sci 83:9759-9763 (1986).
Rhode (b), J. Virology 61:1448-1456 (1987).
Lebovitz et al., J. Virology 58:271-280 (1986).
Carney Vincent L.
Chambers Jasemine C.
The Board of Regents of the University of Nebraska
LandOfFree
Method of controlling the expression of a gene in a cell culture does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of controlling the expression of a gene in a cell culture, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of controlling the expression of a gene in a cell culture will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2098146