Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-11-20
1994-04-19
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352523, 43525233, 4353201, 935 39, 935 40, 935 10, 935 61, 935 72, 935 73, 536 237, C12N 100, C12N 1508, C12N 1531, C12N 1567
Patent
active
053044720
ABSTRACT:
Nucleic acid is provided encoding a molecule having certain variations within the phosphate-binding region of native E. coli PstS. Additionally provided are bacterial cells comprising this nucleic acid under control of the native pstS gene promoter, and optionally further comprising nucleic acid encoding a polypeptide of interest under control of the alkaline phosphatase (AP) promoter. Bacterial cells containing both pstS variant nucleic acid and polypeptide nucleic acid are cultured in a medium at a concentration of inorganic phosphate that at all phases of cell growth is above the level at which the cells are starved for phosphate. Alternatively, the cells are cultured under conditions whereby the concentration of inorganic phosphate in the culture medium is controlled during the production period so that the polypeptide is produced under the control of the partially induced AP promoter.
REFERENCES:
patent: 4703005 (1987-10-01), Nakata et al.
Nakata, A., et al., in Phosphate Metabolism and Cellular Regulation in Microorganisms, Torriani-Gorini et al., Eds., American Society for Microbiology, Washington, D.C., 1987, pp. 150-155.
Luecke, H., et al., Nature, vol. 347, pp. 402-406, Sep. 27, 1990.
Surin, B. P., et al., Journal of Bacteriology, vol. 157, pp. 772-778, Mar. 1984.
Torriani, A., Bioessays, vol. 12, pp. 371-376, 1990.
Worobec, E. A., et al., FEMS Microbiology Letters, vol. 52, pp. 235-238, 1988.
Wanner, J. Cell. Biochem., 51:47-54, (1993), "Gene Regulation by Phosphate in Enteric Bacteria".
Rao et al., J. Bacteriol., 175:74-79, (1993), "Effect of glpT and glpD Mutations on Expression of the phoA Gene in Escherichia coli".
Foglino et al., Mol. Gen. Genet., 210:523-527, (1987), "Deletion Analysis of the Promoter Region of the Escherichia coli pepN gene, a Gene Subject In Vivo to Multiple Global Controls".
Gharbi et al., J. Bacteriol., 163:1191-1195, (1985), "Multiple Controls Exerted on In Vivo Expression of the pepN gene in Escherichia coli: Studies with pepN-lacZ Operon and Protein Fusion strains".
Cooper, Ann. Rev. Microbiol., 38:49-68, (1984), "Metabolism of Methylglyoxal in Microorganisms".
Stock et al., Microbiol. Rev., 53:450-490, (1989), "Protein Phosphorylation and Regulation of Adaptive Response in Bacteria".
Lueke & Quiocho, Nature, "High Specificity of a Phosphate Transport Protein Determined by Hydrogen Bonds", 347:402-406 (1990).
Torriani, Biochim. Biophys. Acta, "Influence of Inorganic Phosphate in the Formation of Phosphatases by Escherichia coli", 38:460-469 (1960).
Ohinagawa et al., "Structure and Function of the Regulatory Genes for the Phosphate Reguion in Escherichia coli", in Phosphate Metabolism and Cellular Regulation in Microorganisms, Torriani-Gorini, eds., American Society for Microbiology, Washington, D.C., 1987, pp. 20-25.
Wanner, "Phosphate Regulation of Gene Expression in Escherichia coli", in Neidharat et al., ed., Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, American Society for Microbiology, Washington, D.C., 1987, pp. 1326-1333.
Torriani, "From Cell Membrane to Nucleotides: The Phosphate Regulon in Escherichia coli", Bioessays, 12(8):371-376 (1990).
Matin et al., "Genetic Basis of Starvation Survival in Nondifferentiating Bacteria", Ann. Rev. Microbiol., 43:293-316 (1989).
Iwakura et al., "Isolation of DNA Fragment Containing phoS Gene of Escherichia coli K-12", J. Biochem., 92:615-622 (1982).
Nagota et al., "Nucleotide Sequence of the phoS Gene, the Structural Gene for the Phosphate-Binding Protein of Escherichia coli", J. Bacteriol., 157(3):909-917 (1984).
Surin et al., "Structural Gene for the Phosphate-Repressible Phosphate-Binding Protein of Escherichia coli Has Its Own Promoter . . . ", J. Bacteriol., 157(3):772-778 (1984).
Kimura et al., "Regulation of the Phosphate Regulon of Escherichia coli . . . ", Mol. Gen. Genet., 215:374-380 (1989).
Levitz et al., "The Effect of the Locus pstB on Phosphate Binding in the Phosphate Specific Transport System of Escherichi coli", Mol. Gen. Genet., 200:118-122 (1985).
St. John & Goldberg, "Effects of Starvation for Potassium and Other Inorganic Ions of Protein Degradation and Ribonucleic Acid Synthesis in Eschericia coli", 143(3):1223-1233 (1980).
Nakata et al., "Genetic and Biochemical Analysis of the Phosphate-Specific Transport System in Escherichia coli", in Phosphate Metabolism and Cellular Regulation in Microorganisms, Torriani Gorini et al., eds., American Society for Microbiology, Washington, D.C. 1987, pp. 150-155.
Bass Steven
Swartz James R.
Genentech Inc.
Hasak Janet E.
Moore William W.
Wax Robert A.
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