Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-09-10
1998-06-09
Rories, Charles C. P.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 25, 435 691, 435 711, 435189, 4353201, 435375, 47 58, 424 932, 424 93461, 424 944, 536 232, 536 237, 536 2371, 800205, 800250, 800255, 800DIG27, 800DIG56, C12N 1563, C12N 1582, A01H 500, A01G 1300
Patent
active
057632456
ABSTRACT:
3-Hydroxysteroid oxidase in combination with Bacillus thuringiensis CryIA(b) or CryIA(c) crystal proteins or polypeptides controls insects, particularly lepidopterans. The combination when applied to larvae of several lepidopterans indicated significant effects on mortality and stunting and indicated responses that were statistically significant over those expected from additive, independent action. Cholesterol oxidase and CryIA(b) crystal protein were also effective against black cutworm neonates when co-expressed in transgenic corn and tested in a detached leaf assay.
REFERENCES:
patent: 5518908 (1996-05-01), Corbin et al.
patent: 5530197 (1996-06-01), Peferoen
patent: 5554369 (1996-09-01), Corbin et al.
patent: 5558862 (1996-09-01), Corbin et al.
Bagdasarian M., Lurz R., Ruckert, B., Franklin F.C.H., Bagdasarian M.M., Frey, J. and Timmis K.N., "Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas, " Gene, 16:237-47, 1981.
Bevan M., et al., "A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation," Nature, 304:184-187, 1983.
Burnette W.N., "Western Blotting: Electrophoretic transfer of proteins from Sodium Dodecyl Sulfate-Polyacrylamidegels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A," Anal. Biochem, 112:195-203, 1981.
Cornelissen B.J.C. et al., "Molecular characterization of messenger RNAs for `pathogenesis-related` proteins 1a, 1b and 1c, induced by TMV infection of tobacco," EMBO Journal, 5:37-40, 1986.
Finney D.J., "Probit Analysis," Cambridge University Press, London, 1964.
Gallo L.L., "Pancreatic sterol ester hydrolase," Methods Enzymol., 71:664-674, 1981.
Herrera-Estrella L., et al., Nature, "Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector," 303:209-213, 1983.
Ishizaki T., Hirayama N., Shinkawa H., Nimi O., Murooka Y, "Nucleotide Sequence of the Gene for Cholesterol Oxidase from a Streptomyces sp," Journal of Bacteriology, 171:596-601, 1989.
Kay R., et al., "Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes," Science, 236:1299-1302, 1987.
Klee H.J., et al., "Vectors for Transformation of Higher Plants," Bio/Technology, 3:637-642, 1985.
Knauf V.C. and Nester E., "Wide host range cloning vectors:A cosmid bank of an Agrobacterium Ti plasmid," Plasmid, 8:45-54, 1982.
Laemmli U.K., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4," Nature, 227:680-685, 1970.
Marrone P.G., Ferri F.D., Mosley T.R., Meinke L.J., "Improvements in laboratory rearing of the southern corn rootworm, Diabrotica undecimpuncta howardiBarber (Coleopter: Chrysomelidae) on an artificial diet and corn" Journal of Economic Entomology, 78:290-293, 1985.
Matsudaira P., "Sequence from picomole guantities of proteins electroblotted onto polyvinylidene difluoride membranes," Journal of Biol. Chem., 262-10035-10038, 1987.
Moore S. and Stein W.H., "Chromatographic determination of amino acids by the use of automatic recording equipment," Methods in Enzymology, 6:819-831, 1963.
Purcell J., Greenplate J.T., and Sammons R.P., "Examination of midgut luminal proeinase activites in six economically important insects," Insect Biochem. Molec. Biol., 22:41-47, 1992.
Schuler M.A., et al., "Closely related families of genes code for the.alpha. and .alpha.' subunits of the soybean 7S storage protein complex," Nucleic Acids Research, 10:8225-8244, 1982.
Smith A.G. and Brooks, C.J.W., "Cholesterol oxidases: Properties and Applications," Journal of Steroid Biochemistry, 7:705-713, 1971.
Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gertner F., Provenzano M.D., Fujimoto E.K., Goeke M.N., Olson B.J., Klenk, D.C. "Measurement of protein using bicinchoninic acid," Analytical Biochemistry, 150:76-85, 1985.
Winter et al., "The inhibition of petunia hsp70 mRNA processing CdCl.sub.2 stress," Mol. Gen. Genet., 221(2):315-319, 1988.
Yanisch-Perron C., Viera J., and Messing J., "Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors," Gene, 33:103-119, 1985.
Corbin David R.
Greenplate John T.
Pershing Jay C.
Purcell John P.
Monsanto Company
Rories Charles C. P.
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