Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2007-09-11
2007-09-11
Mehta, Ashwin (Department: 1638)
Chemistry: molecular biology and microbiology
Vector, per se
C435S419000
Reexamination Certificate
active
10611748
ABSTRACT:
This invention relates to methods of controlling gene silencing using site-specific recombination. A variety of constructs are provided which are useful for conditional or regulated gene silencing in plants, comprising a suite of constitutive, inducible, tissue-specific or developmental stage-specific promoters operably linked to target sequences (TS). Recombinase inversion or excision yields double-stranded TS RNA, which thereby functions to trigger endogenous gene silencing mechanisms. By matching promoters, responsive to various inducers, plant tissues or plant developmental states with the recombinase systems, transcriptional stop fragments or introns and target sequences, gene silencing of virtually any target sequence may be modulated at any plant development stage or in any plant generation. This is especially useful, when genes responsible for gene silencing are down-regulated to permit expression of particular transgenes at levels greater than permitted when gene silencing is activated.
REFERENCES:
patent: 6077992 (2000-06-01), Yadav
patent: 6110736 (2000-08-01), Hodges et al.
patent: WO91/09957 (1991-07-01), None
patent: WO93/01283 (1993-01-01), None
patent: WO97/37012 (1997-10-01), None
patent: WO 00/17365 (2000-03-01), None
patent: WO 01/36595 (2001-05-01), None
Fire, A., RNA-triggered gene silencing, Trends Genet. 15(9): pp. 358-383, 1999.
Sharp, P.A., RNAI and double-strand RNA, Genes Dev., vol. 13: pp. 139-141, 1999.
Sharp et al., RNA Interference, Science, 287, 5482, 2431-3, 2000.
Waterhouse et al., Gene silencing as an adaptive defence against viruses, Nature, 411:834-842, Jun. 14, 2001.
Vaucheret et al., Transcriptional gene silencing in plants: targets, inducers and regulators, TRENDS in Genetics 17(1): pp. 29-35, 2001.
Okamoto et al., Silencing of transposable elements in plants, TRENDS in Plant Sci. 6(11): 527-534, 2001.
Pruss, et al., Plant Viral Synergism: The P{otyviral Genome Encodes a Broad-Range Pathogenicity Enhancer that Transactivates Replication of Heterologous Viruses, Plant Cell 9: pp. 859-888, 1997.
Anandalakshmi et al., A viral suppressor of gene silencing in plants, Proc. Natl. Acad. Sci. USA, 95,(22): 13079-84, 1998.
Brigneti, G. et al., Viral pathogenicity determinants are suppressors of transgene silencing inNicotiana benthamiana, EMBO J., 17(22): 6739-46, 1998.
Kasschau, K. D. and J. C. Carrington, A Counterdefensive Strategy of Plant Viruses: suppressionof Postranscriptional Gene Silencing, Cell, 95(4): 481-70, 1998.
Smith et al., Total silencing by intron-spliced hairpin RNAs, Nature 407: 319, 2000.
De Buck et al., Transgene silencing of invertedly repeated transgenes is released upon deletion of one of the transgenes involved, Plant Mol. Biol. 46: 433, 2001.
Hulvagner et al., A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA, Science 293: 834, 2001.
Odell et al., Seed Specific Gene Activation Mediated by the Cre/lox Site-Specific Recombination System, Plant Physiol. 106: 447-458, 1994.
Russel et al., Directed excision of a transgene from the plant genome, Mol. Gen. Genet. 234: 49-59, 1992.
Craig, The mechanism of Conservative Site-Specific Recombination, Annu. Rev. Genet. 22: 77-105, 1998.
Odell et al., Use of site-specific recombination systems in plants. Homologous Recomb. Gene Silencing Plants, 1994, pp. 219-270, Paszkowski, Jerzy, ed. Kluwer: Dordrecht, Germany.
Zubko et al., Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes, Nature, Biotechnology, 18: 442, 2000.
Groth et al., A phage integrase directs efficient site-specific integration in human cells, Proc. Natl. Acad. Sci. USA 97: 5995, 2000.
Chromosome Engineering inSaccharomyces cerevisiaeby Using a Site-Specific Recombination System of a Yeast Plasmid, H. Matsuzaki et al., J. Bacteriology, 172, 610, 1990.
Onouchi et al., Operation of an efficient site-specific recombination system ofZygosaccharomyces rouxiiin tobacco cells, Nucleic Acid Res., 19: 6373-1991.
Ambros, Dicing Up RNAs, Science 293: 811-813, Aug. 3, 2001.
Vance et al., RNA Silencing in Plants—Defense and Counterdefense, Science 292: 2277, 2001.
Knight et al., A Role for the Rnase III Enzyme DCR-1 in RNA Interference and Germ Line Development inCaenorhabditis elegans, Science vol. 293, Sep. 21, 2001.
Odell et al., Site-directed recombination in the genome of transgenic tobacco, Mol. Gen. Genet. 1990, vol. 223-pp. 369-378.
Russell et al., Tissue-specific expression in transgenic maize of four endosperm promoters from maize and rice, Transgenic Research 6, pp. 157-168, 1997.
Lyznik et al., FLP-mediated recombination of FRT sites in the maize genome, Nucleic Acids Research, 1998, vol. 24, No. 19, pp. 3784-3789.
Holtorf et al., Comparison of different constitutive and inducible promoters for the overexpression of transgenes inArabidopsis thaliana, Plant Molecular Biology vol. 29, pp. 637-648, 1995.
Dalmay et al., AN RNA-DependentRNA Polymerase Gene inArabidopsisis Required for Posttranscriptional Gene Silencing mediated by a Transgene but Not by a Virus, Cell, vol. 101, 543-553, May 26, 2000.
Lloyd et al., Functional expression of the yeast FLP/FRT site-specific recombination system inNicotiana tabacum, Mol. Gen. Genet. 1994, vol. 242: pp. 653-657.
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