Catalyst – solid sorbent – or support therefor: product or process – Solid sorbent – Organic
Patent
1994-04-28
1996-10-01
Pal, Asok
Catalyst, solid sorbent, or support therefor: product or process
Solid sorbent
Organic
502401, 502403, 502159, 502162, 502168, 502172, B01J 2026
Patent
active
055610978
ABSTRACT:
A method is disclosed for controlling the coupling low molecular weight ligands to activated sites on a support in competition with a quencher of the activated sites. Derivatized supports produced by the method have optimal ligate binding without overly dense binding sites or stearic effects. Azlactone-functional supports, especially porous supports such as porous particles, benefit from control of ligand density. A linear relationship exists between the molar ratio of ligand and quencher and the coupled ligand density. Optionally, hydrophilicity of the derivatized support can be determined by selection of quencher relative to selection of ligand.
REFERENCES:
patent: 4212905 (1980-07-01), Tsibris
patent: 4451619 (1984-05-01), Heilmann et al.
patent: 4582875 (1986-04-01), Ngo
patent: 4737560 (1988-04-01), Heilmann et al.
patent: 4871824 (1989-10-01), Heilmann et al.
patent: 4968742 (1990-11-01), Lewis et al.
patent: 5013795 (1991-05-01), Coleman et al.
patent: 5200471 (1993-04-01), Coleman et al.
patent: 5262484 (1993-11-01), Coleman et al.
patent: 5292514 (1994-03-01), Capecchi et al.
patent: 5292840 (1994-03-01), Heilmann et al.
Velander et al., "The Use of Fab-Masking Antigens to Enhance the Activity of Immobilized Antibodies", Biotechnology and Bioengineering, vol. 39, 1013-1023 (1992).
Landgrebe et al., "Preparation of Chromatographic Supports of Variable Ligand Density", Anal. Chem., 58, 1607-1611 (1986).
Wu et al., "Effects of stationary phase ligand density of high performance ion-exchange chromatography of proteins", Journal of Chromatography, 598, 7-13 (1992).
Wirth, H. et al., "Influence of Ligand Density on the Properties of Metal-Chelate Affinity Supports", Analytical Biochemistry, vol. 208, pp. 16-25 (1993).
Gleason Raymond M.
Rasmussen Jerald K.
Bullock In Suk
Griswold Gary L.
Hornickel John H.
Kirn Walter N.
Minnesota Mining and Manufacturing Company
LandOfFree
Method of controlling density of ligand coupled onto supports an does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of controlling density of ligand coupled onto supports an, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of controlling density of ligand coupled onto supports an will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1501940