Chemistry: molecular biology and microbiology – Condition responsive control process
Patent
1996-07-01
1999-03-16
Beisner, William H.
Chemistry: molecular biology and microbiology
Condition responsive control process
435 67, 435166, 4352571, 47 14, C12Q 300
Patent
active
058828494
ABSTRACT:
A control method for operating aqueous Haematococcus spp., such as H. pluvialis, microorganism growth processes is disclosed which can maintain viable growth conditions of this microorganism which have heretofore not been easily reproduced in commercially valuable quantities. The primary control parameters are the degree of turbulence in the aqueous growth medium and the scale of the apparatus relative to the scale of the turbulent eddies in vessels which are partially filled with the aqueous medium directly affect conditions which are required for optimum growth: light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity. These control elements can be cast in terms of the Reynolds number (N.sub.re), pH, temperature, amount of impinging light, and NO.sub.2 concentration, depending upon the operative chlorophyll growth stages of Haematococcus spp. and its photoadaptive stages of producing astaxanthin.
REFERENCES:
patent: 4871551 (1989-10-01), Spenser
patent: 5541056 (1996-07-01), Huntley et al.
"Enhancement and Determination of Astaxanthin Accumulation in Green Alga Haematococcus pluvialis," Sammy Boussiba, et al., Methods in Enzymology, vol. 213, 1992.
"Autotrophic growth and carotenoid production of Haematococcus pluvialis in a 30 liter air lift photobioreactor," Mark Harker, et al., Chemical Abstracts, vol. 125, No. 17, Abstract No. 219715, Oct. 21, 1996, Columbus, Ohio, USA.
Huntley Mark E.
Leonard Alexander B. P.
Niiler Pearn P.
Redalje Donald
Aquasearch, Inc.
Beisner William H.
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