Method of continuously producing ethanol from sugar-containing s

Details Method of continuously producing ethanol from sugar-containing s Method of continuously producing ethanol from sugar-containing s

1988-01-27

1989-10-24

Warren, Charles F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435162, 435813, 435222, G12P 706, G12P 714

Patent

active

048761966

ABSTRACT:
In a method of continuously producing ethanol from sugar-containing substrates by fermentation of sugars by means of a flocculating strain of Zymomonas mobilis under anaerobic conditions and at a pH of from 4.5 to 7 a substrate is led commonly with Zymomonas mobilis cells through at least three fermentation stages without preceding sterilization, a concentration of at least 4% by volume of ethanol is maintained in each fermentation stage, a residence time of the fermentation medium in the entire system of maximally 31/3 h corresponding to a dilution rate of the fermentation medium in the entire system of at least 0.3 h.sup.-1 is adjusted, the Zymomonas mobilis cells are separated by sedimentation after the final fermentation stage, the Zymomonas mobilis cells are recycled into the first fermentation stage, and the ethanol-containing substrate separated from the Zymomonas mobilis cells is drawn off.

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Karsch et al., "Ethanol Production by Zymomonas and Saccharomyces", Applied Microbiology and Biotechnology, Spranger-Vertag, 1983.
Rogers et al., "Ethanol Production by Zymomonas mobilis", Advances in Biochemical Engineering, vol. 23.
W. Kuhlmann et al., "Reaktionstechnische Aspekte der Ethanolproduktion in einer Dreistufigen Ruhrkesselreaktorkaskade Ohne und mit Zellruckfuhrung", Symposium Technische Mikrobiologie, Berlin, (1982).
K. Rosen, "Continuous Production of Alcohol", Process Biochemistry, 5/78, pp. 25-26.
Fein et al., "High Productivity Continuous Ethanol Fermentation with a Flocculating Mutant Strain of Zymomonas mobilis", Biotechnology Letters, vol. 5, No. 1, 1983, pp. 19-24.
Klein et al., "Improvement of Productivity and Efficiency in Ethanol Production with Ca-Alginate Immobilized Zymomonas mobilis", Biotechnology Letters, vol. 5, No. 8, (1983), pp. 497-502.
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