Method of constructing vectors for homologous recombination...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S455000, C536S023100, C536S091000

Reexamination Certificate

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06924146

ABSTRACT:
The present invention provides a novel vector system and thereby a novel method for the simplified construction of recombinant vectors for directed mutagenesis. Said vector system is used to modify the eukaryotic genome, particularly of embryonic stem cells, at precise and predefined loci by the means of homologous recombination. Furthermore, said system finds its usage in the generation of new strategies for gene therapy and in the generation of genetically modified higher eukaryotic organisms.

REFERENCES:
patent: 5487992 (1996-01-01), Capecchi et al.
patent: 5589369 (1996-12-01), Seidman et al.
patent: 5650550 (1997-07-01), Korach et al.
patent: 6090554 (2000-07-01), Woychik
patent: 6090629 (2000-07-01), Woychik
patent: 6528313 (2003-03-01), Le Mouellic
patent: 6528314 (2003-03-01), Le Mouellic
patent: 6537542 (2003-03-01), Treco
Thorsten Storck, et al.Rapid Construction in Yeast of Complex Targeting Vectors for Gene Manipulation in the Mouse. Nucleic Acids Research, vol. 24, No. 22, pp. 4594-4596 (1996).
Krisher, RL et al. Journal of Diary Science 78(6):1282-1288, Jun. 1995.
Nehls, m et al. Biotechniques 17(4):770-775, Oct. 1994.
Storck, T. et al. Nucleic Acid Research 24(22):4594-4595, Nov. 1994.
Seamark, R.F. Reproduction Fertility and Development 6:653-657, Jun. 1995.

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