Method of constructing orchid haploid by treating...

Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se

Reexamination Certificate

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C435S430000, C435S420000, C800S295000, C800S298000, C800S299000

Reexamination Certificate

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07442549

ABSTRACT:
It is intended to provide a pure line plant which is required in constructing a seed propagation variety of orchid. An auxin solution is dropped to unfertilized orchid flowers so as to form seeds based on parthenogenesis. Then these seeds are germinated and haploid plants are selected from orchid plants thus grown. The germinating seeds judges as haploid plants are grown to give pure line plants having doubled chromosomes. Thus, a seed propagation variety of orchid is obtained.

REFERENCES:
patent: 2000-342093 (2000-12-01), None
Xian Sheng Zhang and Sharman D. O'Neill, Ovary and Gametophyte Development Are Coordinately Regulated by Auxin and Ethylene following Pollination, The Plant Cell, vol. 5, pp. 403-418, Apr. 1993.
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Anhthu Q. Bui, et al. “Three 1-Aminocyclopropane-1-Carboxylate Synthase Genes Regulated by Primary and Secondary Pollination Signals in Orchid Flowers”, Plant Physiology, XP-002400721, vol. 116, No. 1, Jan. 1998, pp. 419-428.
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Joseph Arditti, et al. “The Effects of Auxin, Actinomycin D., Ethionine, and Puromycin on Post-Pollination Behavior by Cymbidium (ORCHIDACEAE) Flowers”, American Journal of Botany, Botanical Society of America, XP-008057821, vol. 56, No. 6, 1969, pp. 620-628.

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