Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-11-08
2005-11-08
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C536S023100, C536S023200, C536S024100, C435S026000, C435S069100, C435S189000, C435S190000, C435S471000, C435S252320, C435S232000, C435S193000
Reexamination Certificate
active
06962805
ABSTRACT:
A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
REFERENCES:
patent: 1155300 (1997-07-01), None
patent: 0 771 879 (1997-05-01), None
patent: WO00/18935 (2000-04-01), None
patent: 61-104790 (1986-05-01), None
patent: 63-214189 (1988-09-01), None
patent: 6-502548 (1994-03-01), None
patent: 2708168 (1997-10-01), None
patent: WO 93/03158 (1993-02-01), None
Bormann et al. , GenBank accession No. X59404, 1992.
Eikmanns et al. , GenBank accession No. X66112, 1994.
Nakamura et al. , GenBank accession No. AB025424, Apr. 1999.
Eikmanns et al. , GenBank accession No. X71489, 1995.
Lewin, Genes IV, pp. 225-226, 1990.
Bork , Genome Research, 10:398-400, 2000.
Broun et al. , Science 282:1315-1317, 1998.
Seffernick et al. , J. Bacteriol. 183(8):2405-2410, 2001.
Van de Loo et al. , Proc. Natl. Acad. Sci. 92:6743-6747, 1995.
Baggio et al., GenEMBL accession No. BTU82241, Jan. 11, 1997.
Teller et al., GenEMBL accession No. CLOSGDHG, Jun. 30, 1993.
B. Fournier, et al., “Strength and Regulation of the Different Promoters for Chromosomal β-Lactamases ofKlebsiella oxytoca”, Antimicrobial Agents and Chemotherapy, vol. 43, No. 4, Apr. 1999, p. 850-855.
T. Jeffke, et al., “Mutational Analysis of the CBB Operon (CO2Assimilation) Promoter of Ralstonia Eutropha”, Journal of Bacteriology, vol. 181, No. 14, Jul. 1999, p. 4374-4380.
C. Guerrero, et al., Gene, vol. 138, XP-002285948, pp. 35-41, “Directed Mutagenesis of a Regulatory Palindromic Sequence Upstream from the Brevibacterium Lactofermentum Tryptophan Operon”, 1994.
K. Sano, et al., Gene, vol. 53, XP-002285949, pp. 191-200, “Structure and Function of the TRP Operon Control Regions of Brevibacterium Lactofermentum, a Glutamic-Acid-Producing Bacterium”, 1987.
E.M.T. El-Mansi, “Control of Metabolic Interconversion of Isocitrate Dehydrogenase Between the Catalytically Active and Inactive Forms inEscherichia coli”, FEMS Microbiology Letters, 166, (1998), 333-339.
E. Alvarez-Villafane, et al., “Two NAD+-Isocitrate Dehydrogenase Forms in Phycomyces Blakesleeanus, Induction in Response to Acetate Growth and Characterization, Kinetics, and Regulation of Both Enzyme Forms”, Biochemistry, 1996, 35, 4741-4752.
R.F. Cadenas, et al., Gene, vol. 98, pps. 117-121, “Construction and Characterization of Promoter-Probe Vectors for Corynebacteria Using the Kanamycin-Resistance Reporter Gene,” 1991.
A. Cid, et al. Current Genetics, vol. 12, pps. 105-110, “Replacement of the Promoter of the Yeast Plasma Membrane ATPase Gene by a Galactose-Dependent Promoter and its Physiological Consequences,” 1987.
R.E. Dalbey, et al., The Journal of Biological Chemistry, vol. 260, No. 29, pps. 15925-15931, “Leader Peptidase Catalyzes the Release of Exported Proteins From the Outer Surface of theEscherichia coliPlasma Membrane,” Dec. 15, 1965.
G. Miozzari, et al., Proc. Natl. Acad. Sci., vol. 75, No. 11, pps. 5580-5584, “Naturally Occurring Promoter Down Mutation: Nucleotide Sequence of the TRP Promoter/Operator/Leader Region ofShigella dysenteriae16,” Nov. 1978.
Y. Morinaga, et al., Journal of Biotechnology, vol. 5, pps. 305-312, “Expression ofEscherichia coliPromoters in Brevibacterium Lactofermentum Using the Shuttle Vector pEB003,” 1987.
M. Patek, et al., Microbiology, vol. 142, pps. 1297-1309, “Promoters From Corynebacterium Glutamicum: Cloning, Molecular Analysis and Search for a Consensus Motif,” 1996.
O. Raibaud, et al., Gene, vol. 29, pps. 231-241, “A Technique for Integrating any DNA Fragment Into the Chromosome ofEscherichia coli” 1984.
M. Rosenberg, et al., Ann. Rev. Genet. vol. 13, pps. 319-353, “Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription,” 1979.
H. Shimotsu, et al., Gene, vol. 43, pps. 85-94, Construction of a Single-Copy Integration Vector and its use in Analysis of Regulation of the TRP Operon ofBacillus subtilis.
S. Sugimoto, et al., Journal of Biology, vol. 5, pps. 237-253, “Hyperproduction of Phenylalanine byEscherichia coli: Application of a Temperature-Controllable Expression Vector Carrying the Repressor-Promoter System of Bacteriophage Lambda,” 1987.
P. Youderian, et al., Cell, vol. 30, pps. 843-853, “Sequence Determinants of Promoter Activity,” Oct. 1982.
T.J. Zupancic, et al., FEMS Microbiology Letters, vol. 131, pps. 121-126, “Isolation of Promoters FromBrevibacterium flavumStrain MJ233C and Comparison of Their Gene Expression Levels inB. FlavumandEscherichia coli,” 1995.
Asakura Yoko
Ito Hisao
Kanno Sohei
Kimura Eiichiro
Kurahashi Osamu
Guest Cemaik Shelly
Prouty Rebecca E.
Ramirez Delia M.
LandOfFree
Method of constructing amino acid producing bacterial... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of constructing amino acid producing bacterial..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of constructing amino acid producing bacterial... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3506066