Method of constructing a synthetic polynucleotide

Details Method of constructing a synthetic polynucleotide Method of constructing a synthetic polynucleotide

2007-06-12

2007-06-12

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091400, C435S371000

Reexamination Certificate

active

10305765

ABSTRACT:
A method of constructing a synthetic polynucleotide, the method including selecting a first codon of a parent polynucleotide that encodes a polypeptide for replacement with a synonymous codon, wherein the synonymous codon is selected on the basis that it exhibits a higher translational efficiency in an epithelial cell of a mammal than the first codon in a comparison of translational efficiencies of codons in test cells of the same type as the epithelial cell; and replacing the first codon with the synonymous codon to construct the synthetic polynucleotide.

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