Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1994-01-28
1995-10-17
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 18, 435 21, 435 25, 435 26, C12Q 100, C12Q 134, C12Q 142, C12Q 126
Patent
active
054590329
ABSTRACT:
A method and apparatus for performing an enzyme spot test without traditional instrumentation is disclosed. The method utilizes various reagents to allow the visual determination of the presence of an enzyme in a sample above a predetermined critical level. The enzyme reacts with the reagents producing a product according to a reaction which is timed by a chemical fuse. At the end of the reaction, a colored by-product is visible, the color intensity indicating that the enzyme occurs in the sample in quantities above the critical level.
REFERENCES:
patent: 4066403 (1978-01-01), Bruschi
patent: 4446231 (1984-05-01), Self
patent: 4782016 (1988-11-01), Norton
Rawn David, Biochemistry, pp. 235-236, 1983 Harper & Row.
Fisher Scientific Catalog 1988, #1099, 1101, 1103, 1109.
Elser Robert C.
Valenta Jane N.
Weber Stephen G.
Gitomer Ralph
University of Pittsburgh
Wityshyn Michael G.
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