Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2001-03-22
2010-11-16
Chen, Stacy B (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C424S207100, C435S239000
Reexamination Certificate
active
07833700
ABSTRACT:
A method of concentrating and recovering an enzyme activity from enveloped viruses present in a biological sample, is described. The method comprises contacting the biological sample in a first buffer solution with a virus-binding matrix, such as an anion exchanger matrix, to attach virus particles present in the sample to the matrix, washing the matrix carrying the virus particles with a second buffer solution to remove components interfering with viral enzyme activity, lysing the immobilized virus particles in a third buffer solution and recovering the concentrated viral enzyme activity from the third buffer solution. Additionally, a commercial package containing written and/or data carrier instructions for performing laboratory steps for concentration and recovery of an enzyme activity from enveloped viruses present in a biological sample and at least one component necessary for the assay, is disclosed.
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O'Neil, Virus Harvesting and Affinity Based Liquid Chromatography, Feb. 1993, Bio/Technology vol. 11, pp. 173-178.
Gatu Tommy
Gronowitz Simon
Källander Clas
Malmsten Anders
Pettersson Ingvar
Bacon & Thomas, PLL
Cavidi AB
Chen Stacy B
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