Method of cloning modified streptomycetes DNA

Details Method of cloning modified streptomycetes DNA Method of cloning modified streptomycetes DNA

1984-09-25

1987-12-01

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351721, 4351723, 435253, 435886, 435320, 935 31, 935 55, 935 56, 935 57, 935 58, 935 73, 935 75, 935 79, 935 80, C12P 1934, C12N 1500, C12N 120, C12N 100, C12R 1465

Patent

active

047104660

ABSTRACT:
A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero-specific hosts. The transformants can then be screened for clones containing genes of interest.

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