Method of cloning bovine embryos

Details Method of cloning bovine embryos Method of cloning bovine embryos

1993-03-15

1995-09-26

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352401, 4352402, 800 2, 800DIG4, 800DIG6, C12N 1500, C12N 500

Patent

active

054533660

ABSTRACT:
An improved method of cloning mammalian embryos by transferring a nucleus from a donor mammalian embryo to an enucleated recipient oocyte and culturing the nuclear transferred bovine embryo in vitro is described. The improvement includes positioning the donor embryo nucleus adjacent to the enucleated recipient oocyte, and maintaining the nucleus and oocyte in a maintenance medium for a time sufficient to mature the cytoplasm of the enucleated recipient oocyte prior to fusing the donor nucleus to the recipient oocyte.

REFERENCES:
patent: 4994384 (1991-02-01), Prather et al.
patent: 5057420 (1991-10-01), Massey
patent: 5096822 (1992-03-01), Rosenkrans
Bavister et al., 1983, "Development of Preimplantation Embryos of the Golden Hamster in a Defined Culture Medium, " Biol. Reprod., 28:235.
Bondioli, K. R. et a.. 1990, "Production of Identical Bovine Offspring by Nuclear Transfer," Theriogenology, vol. 13(1):165-174.
Briggs and King, 1952, "Transplantation of Living Nuclei from Blastula Cells into Enucleated Frogs' Eggs, " Zoology 38:455-463.
Briggs, R., et al., 1964, "Transplantation of Nuclei of Various Cell Types from Neurulae of the Mexican Axolotl (Ambystoma mexicanum)," Develop. Biol. 10:233.
Comandon and de Fonbrune, 1939, "Greffe Nucleaire Totale, Simple ou Multiple, Chez une Amibe," Soc. Biol. 130:744.
Critzer, E. S. and N. L. First, 1986, "Use of Flourescent Stain for Visualization of Nuclear Material in Living Oocytes and Early Embryos," Stain Technology, 61(1) :1-5.
Eyestone, et al., 1987, "Culture of One-and Two-Cell Bovine Embryos to the Blastocyst Stage in the Ovine Oviduct", Theriogenology, 28:1-7.
Elsdale et al., 1960, "A Description of the Technique for Nuclear Transplantation in Xenopus laevis," J. Embryol. Exp. Morph., 8(4):437-444.
First and Prather, 1991, "Genomic Potential in Mammals," Diffeerentiation, 48:1-8.
Graham, C. F., 1969, "The Fusion of Cells with One and Two Cell Mouse Embryos," Wistar Inot. Symp. Monogr., 9:19.
Illmensee, K. and P. C. Hoppe, 1981, "Nuclear Transplantation in Mus musculus: Development Potential of Nuclei from Preimplantation Embryos," Cell 23:9.
Kono, T., et al., 1991, "Development of Enucleated Mouse Oocytes Reconstituted with Embryonic Nuclei," J. Reprod. Fert., 93:165-172.
McGrath, J. and D. Solter, 1983, "Nuclear Transplantation in the Mouse Embryo by Microsurgery and Cell Fusion," Science, 220:1300.
McGrath, J. and D. Solter, 1984, "Inability of Mouse Blastomere Nuclei Transferred to Enucleated Zygotes to Support Development In Vitro," Science, 226:1317-1319, 1984.
Navara, C. S. et al., 1992, "Timing of Polarization in Bovine Embryos and Developmental Potential of Polarized Blastomeres," (Abstract) Proc. Soc. Study Reprod., North Carolina State University (Jul. 12-15, 1992), p. 71.
Parrish, J. J. et al., 1988, "Capacitation of Bovine Sperm by Heparin," Biol. Reprod. 38, 1171-1180.
Prather, R. S. et al., 1987, Biol. Reprod., 37:859-866.
Prather, R. S. et al., 1989, Biol. Reprod., 41:414-418.
Robl, J. M. et al., 1986, "Nuclear Transplantation in Mouse Embryos: Assessment of Recipient Cell Stage," Biol. Reprod., 34:733-739.
Sirard et al., 1988, Biol. Reprod., 39:546-552.
Smith, L. C. and I. Wilmut, 1989, "Influence of Nuclear and Cytoplasmic Activity on the Development in vivo of Sheep Embryos after Nuclear Transplantation," Biol. Reprod., 40:1027-1035.
Stice, S. L., and J. M. Robl, 1988, Biol. Reprod., 39:657-664.
Surani, M.A.H. et al., 1986, "Nuclear Transplantation in the Mouse: Heritable Differences Between Paternal Genomes after Activation ofthe Embryonic Genome." Cell, 45:127-136.
Tsunoda, Y. et al., 1987, J. Exp. Zool., (242:147-151).
Willadsen, S. M., 1986, Nature, 320:63-65.

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