Method of cleaving DNA

Details Method of cleaving DNA Method of cleaving DNA

1990-12-14

1993-09-28

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435199, C12P 1934, C12N 922

Patent

active

052486001

ABSTRACT:
A method of cleaving substrate DNA with a restriction enzyme, wherein the substrate DNA is resistant to cleavage by the restriction enzyme, is disclosed. The method comprises co-incubating the substrate DNA and the restriction enzyme with an activating DNA sequence. The activating sequence comprises an oligonucleotide comprised of the restriction enzyme recognition site and cleavage permissible flanking sequences joined directly to both the 5' and 3' ends of the recognition site. Exemplary restriction enzymes which may be used in practicing the present invention include Nae I, BspM I, Hpa II, Nar I, and Sac II.

REFERENCES:
T. Gingeras and J. Brooks, Proc. Natl. Acad. USA 80, 402-406 (1983).
D. Kruger et al., Nucleic Acids Research 16, No. 9, 3997-4008 (1988).
C. Pein et al., FEBS Letters 245, No. 1,2, 141-144 (1989).
Conrad M. et al. (1989) proc. Natl. Acad. Sci., USA 86(24), 9707-9711.

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