Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition
Patent
1993-10-01
1995-07-18
Housel, James C.
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Preparation composition
436166, 436172, 436174, 436800, 436805, G01N 130
Patent
active
054340810
ABSTRACT:
A method for leukocyte classification by flow cytometry that uses a first fluid that is hypotonic acidic fluorescent dye solution and a second fluid that is a solution that changes the osmolarity and pH of the first fluid is disclosed. The first fluid to be used in the method incorporates not only the first and second dyes but also the third dye and this insures that even leukocytes with damaged cell membrane can be distributed as entities distinct from other leukocyte types on a two-dimensional plot. And, hence, high precision of leukocyte classification is assured even in the case of assaying blood that contains damaged cells as a result of prolonged standing at room temperature after sampling.
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Housel James C.
Le Long V.
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