Method of characterizing fluorescent molecules or other...

Radiant energy – Luminophor irradiation

Reexamination Certificate

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C250S459100

Reexamination Certificate

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06927401

ABSTRACT:
A method for characterizing fluorescent molecules or other particles in samples comprising the steps of:a) monitoring fluctuating intensity of fluorescence emitted by the molecules or other particles in at least one measurement volume of a non-uniform spatial brightness profile by measuring numbers of photon counts in primary time intervals by a single or more photon detectors,b) determining at least one distribution of numbers of photon counts, {circumflex over (P)}(n), from the measured numbers of photon counts,c) determining physical quantities characteristic to said particles by fitting the distribution of numbers of photon counts {circumflex over (P)}(n), wherein the fitting procedure involves calculation of a theoretical distribution function of the number of photon counts P(n) through its generating function, defined asG⁡(ξ→)=∑n⁢ξ→n⁢P⁡(n).

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Hong Qian et al., “On the Analysis of High Order Moments of Fluorescence Fluctuations”, vol. 57, pp. 375-380, Feb. 1990.

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