Method of chain combination

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence

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530333, C07K 710

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active

048352519

ABSTRACT:
Production of human relaxin or novel human relaxin analogs by combination of a human relaxin A-chain and B-chain comprises combining the reduced form of the human relaxin A-chain and the reduced form of the human relaxin B-chain in an aqueous medium having a pH of about 7.0 to 12 at room temperature, under conditions that are mildly denaturing for the relaxin B-chain such that the human relaxin or novel human relaxin analog can be formed.

REFERENCES:
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patent: 4656249 (1987-04-01), Florey
patent: 4758516 (1988-07-01), Hudson et al.
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Johnston et al., Ninth American Peptide Symposium, Abstracts, Jun. 23-28, 1985, P-MTu-118, p. 109.
Johnston et al., Ninth American Peptide Symposium, Poster, Jun. 23-28, 1985.
Tregear et al., Biology of Relaxin and Its Role in the Human, Bigazzi et al., Eds., Proceedings of the 1st International Conference on Human Relaxin, Florence, Sep. 30-Oct. 2, 1982, pp. 42-55.
Blundell, T. et al., in Biology of Relaxin and Its Role in the Human, (Exc. Med., Amster.) pp. 14-21, Bigazzi, et al. (eds) 1983.
Busse, W. D. and Carpenter, F. H. Biochemistry, 15: 1649-1657 (1976).
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Hudson, P. et al., Nature, 301: 628-631 (1983).
James, R. et al., Nature, 267: 544 (1977).
Johnston, P. D. et al., in Peptides: Structure & Function, Proc. 9th American Peptide Sympos. (Pierce Chem. Co.), Deber et al. (ed) 1985.
Katsoyannis, P. G. et al., Biochemistry, 6: 2656-2668 (1967).
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Tregear, G. et al., in Relaxin; (Elsevier, N.Y.), Bryant-Greenwood et al. (eds), 1981, pp. 151-164 "The Synthesis of Peptides with Relaxin Activity".
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