Method of cell detection

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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10257493

ABSTRACT:
A method of distinguishing between living and dead cells in a sample, the method includes: (a) contacting the sample with a viability probe which modifies the nucleic acid of dead cells within the sample; and (b) detecting nucleic acid from the cells in the sample, wherein detection involves binding of one or more oligonucleotide probes to a target sequence within the nucleic acid. A method of detecting cells in a sample, the method includes: (a) contacting the sample with a viability probe which labels the nucleic acid of dead cells within the sample; (b) separating the nucleic acid from the cells into labelled and non-labelled fraction; and (c) detecting the nucleic acid in one or both of the fractions.

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