Method of capillary electrophoresis

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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2041828, B01D 5702, B01D 6142, C25B 100

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active

051475170

ABSTRACT:
The method of capillary electrophoresis of the present invention is carried out using an electrophoretic buffer containing an ungelled agarose polymer, wherein the agarose polymer concentration may be constant or may be changed on a time basis.
The method of the present invention permits efficient fractional assay with high reproductibility. Particularly, the mode using the apparatus of the present invention in which the agarose polymer concentration is changed on a time basis is suitable for fractional assay of DNA and proteins of a wide range of size because an electrophoretic buffer having an ideal composition for separation is supplied constantly.

REFERENCES:
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The Use of Liquid Polyacrylamide in Electrophoresis, Hans-Joachim Bode, Zoologiches Institut der Univeritat Heidelberg, 6900 Heidelberg.
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