Method of calibrating ligand specificity

Details Method of calibrating ligand specificity Method of calibrating ligand specificity

2011-04-12

2011-04-12

Gabel, Gailene R (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S006120, C435S007200, C435S973000, C436S501000, C436S517000, C436S518000, C436S523000, C436S528000, C436S008000, C436S034000, C436S177000

Reexamination Certificate

active

07923211

ABSTRACT:
A method to determine specificity of ligand binding includes comparing a solid phase carrier first extract obtained by pre-treating a sample with a ligand-immobilized solid phase carrier and a solid phase carrier second extract obtained by treating the pretreated sample again with a ligand-immobilized solid phase carrier in terms of the proteins contained therein, and identifying a protein whose content is remarkably decreased in the second extract compared to the first extract, in order to solve 1) the problem of the solubility of subject ligand, 2) the problem of the non-specific protein-denaturing effect of the subject ligand added, and the like, in antagonism experiments in target search using an affinity resin.

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