Method of C-terminal peptide sequencing

Details Method of C-terminal peptide sequencing Method of C-terminal peptide sequencing

1989-12-21

1991-09-17

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

530345, 530402, G01N 3368

Patent

active

050495070

ABSTRACT:
A method of C-terminal peptide sequencing. The peptide is reacted with a mixed anhydride of isothiocyanic acid and a carboxylic, carbonic, or sulfonic acid, under basic conditions, to produce a C-terminal peptidyl thiohydantoin. The C-terminal amino acid can be identified by cleaving the thiohydantoin from the peptide and identifying the free amino acid thiohydantoin.

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Miller, M. J., et al., "The Chemistry of a Method for the Determination of Carboxyl-terminal Residues in Peptides" (1987), J. Org. Chem. vol. 42, pp. 1750-1761.
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