Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2006-09-01
2011-12-13
Bell, Kent L (Department: 1661)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
Reexamination Certificate
active
08076537
ABSTRACT:
Methods of breeding germinable transgenic broadleaved tree species are disclosed which include the following steps: (1) the main stem of a tree is cutoff at the height of 50-160 cm above the ground; (2) the transection is dipped with 0.3-0.5 OD600of a suspension of recombinantAgrobacteriumcontaining exogenous target genes for 3 minutes to 24 hours; (3) selective breeding is performed on the third day after infection by dipping the transection infected by recombinantAgrobacteriumin step 2 with antibiotics having lethal effect on theAgrobacteriumevery other 1-2 days to obtain transgenic regeneration plants. In the practical application, the method will have the following active effects: (1) it accelerates the transfer of good exogenous genes to germinable tree species, particularly poplar and facilitates the improvement of varieties thereof; (2) it provides a new way of asexual propagation of germinable broadleaved tree species and increases the productivity and the level of intensive cultivation of germinable broadleaved tree species, so it can be used to solve the ecological problems such as vegetation restoration and to meet the requirement of the market for wood.
REFERENCES:
patent: 6255559 (2001-07-01), Cheah
patent: 2003/0192076 (2003-10-01), Kaplan et al.
patent: 1398511 (2003-02-01), None
patent: 1582641 (2005-02-01), None
patent: 1724654 (2006-01-01), None
patent: WO 2005/032241 (2005-04-01), None
International Search Report dated Nov. 29, 2006.
Han Suying
Qi Liwang
Wang Jianhua
Zhang Shougong
Bell Kent L
Knobbe Martens Olson & Bear LLP
The Research Institute of Forestry, Chinese Academy of Forestry
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