Method of blending an anticoagulant

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preservative – buffer – anticoagulant or diluent

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436 69, 436176, 514 56, 536 21, 128760, 128763, 128765, 128770, 128771, G01N 3386

Patent

active

055523234

ABSTRACT:
A blended heparin salt for use as an anticoagulant in a blood sample, which substantially eliminates a skew in a determined amount of ionized calcium imparted by the heparin in the blood sample. The blended heparin salt comprises a proportional blend of a zinc salt of heparin and a lithium salt of heparin the salts in a ratio of 1-to-a-fractional-value-less-than-one of the zinc salt of heparin to the lithium salt of heparin by weight based on the USP potency of the salts, the fractional value determined by the ratio of the zinc available per unit of USP potency in the zinc salt of heparin to a predetermined amount of zinc per USP potency to be provided by the blended heparin salt. Methods of blending the proportional amounts of the zinc salt and the lithium salt of heparin are disclosed.

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Lyon et al., Evaluation of Dry Lithium Heparin and Zinc heparin anticoagulants for Whole Blood Ionized Calcium Measurements, Clin. Chem, vol. 39, No. 6, 1993; pp. 1175-1176.
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